Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep

Full text
Author(s):
Zafalon, Luiz F. [1] ; Cunha, Maria L. R. S. [2] ; Brandao, Humberto M. [3] ; Mosqueira, Vanessa C. F. [4] ; Santana, Raul C. M. [1] ; Barioni Junior, Waldomiro [1] ; Martins, Katheryne B. [2] ; Pilon, Lucas E. [5]
Total Authors: 8
Affiliation:
[1] Embrapa Pecuaria Sudeste, Rodovia Washington Luiz Km 234, Cx Postal 339, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Estadual Paulista, UNESP, Inst Biociencias, BR-18618970 Botucatu, SP - Brazil
[3] Embrapa Gado Leite, Rua Eugenio do Nascimento 610, BR-36038330 Juiz De Fora, MG - Brazil
[4] Univ Fed Ouro Preto, Rua Costa Sena 171, BR-35400000 Ouro Preto, MG - Brazil
[5] Univ Estadual Paulista, UNESP, Programa Posgrad Med Vet Prevent, Via Acesso Prof Paulo Donato Castellane, BR-14884900 Jaboticabal, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Pesquisa Veterinária Brasileira; v. 38, n. 4, p. 579-585, APR 2018.
Web of Science Citations: 0
Abstract

ABSTRACT: Coagulase-negative Staphylococcus spp. (CNS) are the main microorganisms involved in ovine mastitis. Treatment at the end of lactation can contribute towards cure and prevention of subclinical cases during the subsequent lactation. However, virulence factors and resistance mechanisms presented by CNS can decrease cure rates. The aims of the study were to identify the species of CNS in milk of mastitic ewes with and without antimicrobial treatment, and to investigate the presence of genes relating to resistance of β-lactam antimicrobials, formation of biofilms, production of enterotoxins and production of the toxic shock syndrome toxin. Cases of failure in the treatment were related with the presence/absence of the respective genes. Sixty sheep were divided into three groups: G1, without treatment; G2, animals treated via the intramammary route with 100mg of cloxacillin during drying off; and G3, sheep treated via the intramammary route with 50 mg of nanoparticulate cloxacillin. Milk samples were gathered during drying off and 15 and 30 days after the parturition of the subsequent lactation. The analyses to identify the species of CNS were carried out by means of the internal transcribe spacer technique and the investigation of the genes responsible for the virulence factors and resistance to oxacillin was performed using the polymerase chain reaction (PCR) technique. No sample was positive for the mecA gene. The only gene relating to production of enterotoxins was sec. Among the genes relating to production of biofilm, icaD was the only one identified in the three experimental groups. Staphylococcus warneri was the main species of CNS isolated during the pre and post-partum periods of the sheep. The species carrying genes relating to production of enterotoxins and biofilms were present in uncured sheep. (AU)

FAPESP's process: 12/23044-0 - Relationship of genotypic characteristics of coagulase-negative Staphylococcus with cure rates after antimicrobial treatment of ovine mastitis
Grantee:Luiz Francisco Zafalon
Support Opportunities: Regular Research Grants