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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Pharmacological treatment with galectin-1 protects against renal ischaemia-reperfusion injury

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Author(s):
Carlos, Carla P. [1, 2] ; Silva, Analice A. [1] ; Gil, Cristiane D. [3] ; Oliani, Sonia M. [3, 1]
Total Authors: 4
Affiliation:
[1] Sao Paulo State Univ, UNESP, Inst Biociencias Letras & Ciencias Exatas, Dept Biol, Sao Jose Do Rio Preto, SP - Brazil
[2] FACERES Sch Med, Dept Med, Sao Jose Do Rio Preto, SP - Brazil
[3] Univ Fed Sao Paulo, UNIFESP, Dept Morphol & Genet, Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 8, JUN 22 2018.
Web of Science Citations: 2
Abstract

Galectin-1 protein (GAL-1) has important anti-inflammatory properties, but related pharmacologic approaches to effectively treat or prevent renal ischaemia and reperfusion injury are highly limited. Here, we investigated the effect of GAL-1 in a renal ischaemia-reperfusion injury rat model and an in vitro hypoxia-reoxygenation model with a proximal renal tubular epithelial cell line. In vivo, pretreatment with GAL-1 attenuated the renal parameters changed by ischaemia-reperfusion/hypoxiareoxygenation, with recovery of renal function, protecting against influx of leukocytes, cell death and oxidative stress. Ischaemia-reperfusion/hypoxia-reoxygenation was also associated with increased renal endogenous expression of GAL-1 and intercellular adhesion molecule 1 (ICAM-1) plus augmented levels of proinflammatory cytokines IL-1 beta, TNF-alpha and MCP-1 and decreased anti-inflammatory IL-10 in urine, all of which were abrogated by GAL-1 treatment. In vitro studies demonstrated renal tubular epithelial cells as an important source of GAL-lduring hypoxia-reoxygenation and confirmed the protective effects of exogenous GAL-1 through downregulation of proinflammatory cytokine release by proximal renal tubular epithelial cells. Collectively, our findings confirm the important antiinflammatory role of GAL-1 in kidney ischaemia and reperfusion injury and indicate its promising use as a therapeutic approach. (AU)

FAPESP's process: 16/02012-4 - Evaluation of the immunomodulatory activity of annexin A1 protein in the regulation of inflammatory disorders of the gastrointestinal system: studies in vivo and in vitro experimental models
Grantee:Sonia Maria Oliani
Support type: Regular Research Grants
FAPESP's process: 11/22113-6 - Action mechanism of the anti-inflammatory galectin 1 protein in renal injury induced by the ischemia and reperfusion process: in vivo and in vitro analyses.
Grantee:Carla Patrícia Carlos
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 15/09858-3 - Mechanisms of action of the galectin-1 and -3 proteins in the allergic inflammation: Study in in vivo and in vitro experimental models and in allergic patients
Grantee:Cristiane Damas Gil
Support type: Regular Research Grants