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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Mast cell heterogeneity and anti-inflammatory annexin A1 expression in leprosy skin lesions

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Costa, Mauricio B. [1] ; Mimura, Kallyne K. O. [2] ; Freitas, Aline A. [1] ; Hungria, Emerith M. [1] ; Sousa, Ana Lucia O. M. [1] ; Oliani, Sonia M. [2] ; Stefani, Mariane M. A. [1]
Total Authors: 7
[1] Univ Fed Goias, Trop Pathol & Publ Hlth Inst, Rua 235 S-N Setor Univ, BR-74605050 Goiania, Go - Brazil
[2] Sao Paulo State Univ, UNESP, Dept Biol, Inst Biociencias Letras & Ciencias Exatas, Sao Jose Do Rio Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Microbial Pathogenesis; v. 118, p. 277-284, MAY 2018.
Web of Science Citations: 0

Mast cells (MCs) have important immunoregulatory roles in skin inflammation. Annexin A1 (ANXA1) is an endogenous anti-inflammatory protein that can be expressed by mast cells, neutrophils, eosinophils, monocytes, epithelial and T cells. This study investigated MCs heterogeneity and ANXA1 expression in human dermatoses with special emphasis in leprosy. Sixty one skin biopsies from 2 groups were investigated: 40 newly diagnosed untreated leprosy patients (18 reaction-free, 11 type 1 reaction/T1R, 11 type 2 reaction/T2R); 21 patients with other dermatoses. Tryptase/try + and chymase/chy + phenotypic markers and toluidine blue stained intact/degranulated MC counts/mm(2) were evaluated. Try(+)/chy(+) MCs and ANXA1 were identified by streptavidin-biotin-peroxidase immunostaining and density was reported. In leprosy, degranulated MCs outnumbered intact ones regardless of the leprosy form (from tuberculoid/TT to lepromatous/LL), leprosy reactions (reactional/reaction-free) and type of reaction (T1R/T2R). Compared to other dermatoses, leprosy skin lesions showed lower numbers of degranulated and intact MCs. Try(+) MCs outnumbered chy(+) in leprosy lesions (reaction-free/reactional, particularly in T2R), but not in other dermatoses. Compared to other dermatoses, ANXA1 expression, which is also expressed in mast cells, was higher in the epidermis of leprosy skin lesions, independently of reactional episode. In leprosy, higher MC degranulation and differential expression of try(+)/chy(+) subsets independent of leprosy type and reaction suggest that the Mycobacterium leprae infection itself dictates the inflammatory MCs activation in skin lesions. Higher expression of ANXA1 in leprosy suggests its potential anti-inflammatory role to maintain homeostasis preventing tissue and nerve damage. (AU)

FAPESP's process: 12/21603-2 - Tissue bioengineering: evaluate skin xenograft using porcine acellular matrices and effect of annexin A1 protein as a therapeutic approach in the regenerative and wound healing processes
Grantee:Sonia Maria Oliani
Support Opportunities: Regular Research Grants
FAPESP's process: 12/13041-4 - Evaluation of skin heterologous graft using acellular matrices obtained from porcine skin and annexin A1 action as anti-inflammatory / immunosuppressive agent.
Grantee:Kallyne Kioko Oliveira Mimura
Support Opportunities: Scholarships in Brazil - Doctorate