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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Changes in the levels of acute-phase protein and other serum protein fractions in Santa Ines ewes fed with a high-concentrate diet

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Author(s):
Girardi, Annita Morais [1] ; Sabes, Amanda Festa [1] ; Fagliari, Jose Jurandir [1] ; da Silva, Paulo Cesar [1] ; De Oliveira, Joao Ademir [2] ; Marques, Luiz Carlos [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Paulista, UNESP, Dept Clin & Cirurgia Vet, Fac Ciencias Agr & Vet, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Sao Paulo - Brazil
[2] Univ Estadual Paulista, UNESP, Dept Ciencias Exatas, Fac Ciencias Agr & Vet, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Small Ruminant Research; v. 162, p. 34-38, MAY 2018.
Web of Science Citations: 0
Abstract

The aim of this study was to evaluate the effects of subacute ruminal acidosis (SARA) on total serum protein (TP), plasma fibrinogen (Fb), and serum protein fractions in seven hay-adapted Santa Ines ewes fed with a high concentrate diet for 133 days. Blood was extracted, before feeding, by external jugular venipuncture into tubes with ethylenediaminetetraacetic acid dipotassium anticoagulant, to evaluate the Fb concentration by heat precipitation, and into plain tubes, for TP analysis by the Biuret method and for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE obtained the following proteins: immunoglobulin A (IgA) and G (IgG); ceruloplasmin (Cp); transferrin (TO; albumin; alpha(1)-antitrypsin (alpha(1)AT); haptoglobin (Hp); alpha(1)-acid glycoprotein (alpha(1)AG); and 140, 101, 95, 46, 36, 34, 31, and 23 kDa molecular weight proteins (MWP). Associations among variables and time were evaluated using regression analysis. Fb concentrations did not change (P > 0.05); IgA, alpha 1AT, and Hp levels increased, while Cp decreased throughout the period. Mean Tf levels increased, reaching the highest value on the 72nd day. The other protein levels oscillated during the study. A positive correlation, measured using Pearson's coefficient, was found between TP and IgG levels (r = 0.77; P < 0.0001). IgA, Hp, Tf, and alpha 1AT showed greater potential for use in the diagnosis of SARA. The occurrence of proteins with unknown functions, but that have been highly influenced by the high-grain diet, increases the possibility of identifying inflammatory markers for SARA. (AU)

FAPESP's process: 12/09111-7 - Study of laminitis in sheep with ruminal lactic acidosis experimentally induced
Grantee:Annita Morais Girardi
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/09220-0 - Study of laminitis in sheep with ruminal lactic acidosis experimentally induced
Grantee:Luiz Carlos Marques
Support type: Regular Research Grants