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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Hippocampal microRNA-mRNA regulatory network is affected by physical exercise

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Author(s):
Fernandes, Jansen [1] ; Vieira, Andre Schwambach [2] ; Kramer-Soares, Juliana Carlota [3] ; Da Silva, Eduardo Alves [1] ; Lee, Kil Sun [4] ; Lopes-Cendes, Iscia [5] ; Arida, Ricardo Mario [1]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Paulo UNIFESP, Dept Physiol, Sao Paulo, SP - Brazil
[2] Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, Campinas, SP - Brazil
[3] Univ Fed Sao Paulo UNIFESP, Dept Psychobiol, Sao Paulo, SP - Brazil
[4] Univ Fed Sao Paulo UNIFESP, Dept Biochem, Sao Paulo, SP - Brazil
[5] Univ Campinas UNICAMP, Sch Med Sci, Dept Med Genet, Campinas, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS; v. 1862, n. 8, p. 1711-1720, AUG 2018.
Web of Science Citations: 2
Abstract

Background: It is widely known that physical activity positively affects the overall health and brain function. Recently, microRNAs (miRNAs) have emerged as potential regulators of numerous biological processes within the brain. These molecules modulate gene expression post-transcriptionally by inducing mRNA degradation and inhibiting the translation of target mRNAs. Methods: To verify whether the procognitive effects of physical exercise are accompanied by changes in the activity of miRNA-mRNA network in the brain, differential expression analysis was performed in the hippo campus of control (CTL) and exercised (Ex) rats subjected to 4 weeks of treadmill exercise. Cognition was evaluated by a multiple trial inhibitory avoidance (MTIA) task and Illumina next-generation sequencing (NGS) was used for miRNA and mRNA profiling. Results: Exercise improved memory retention but not acquisition in the MTIA task. It was observed that 4 miRNAs and 54 mRNAs were significantly altered in the hippocampus of Ex2 (euthanized 2 h after the last exercise bout) group when compared to CTL group. Bioinformatic analysis showed an inverse correlation between 3 miRNAs and 6 target mRNAs. The miRNAs miR-129-1-3p and miR-144-5p were inversely correlated to the Igibp5 and Itm2a, respectively, and the miR-708-5p presented an inverse correlation with Cdkn1a, Per2, Rt1-A2. Conclusion: The exercise-induced memory improvements are accompanied by changes in hippocampal miRNA-mRNA regulatory network. (AU)

FAPESP's process: 13/13725-3 - Influence of aerobic exercise on the expression and distribution of microRNAs in the hippocampal formation of adult rats
Grantee:Jansen Fernandes
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 11/50680-2 - Multimodal investigation of epileptogenesis with emphasis in the implementation of new animal models and new tools
Grantee:Iscia Teresinha Lopes Cendes
Support type: Research Projects - Thematic Grants