Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The Xenon Test Chamber Q-SUN (R) for testing realistic tolerances of fungi exposed to simulated full spectrum solar radiation

Full text
Author(s):
Dias, Luciana P. [1] ; Araujo, Claudineia A. S. [2] ; Pupin, Breno [2] ; Ferreira, Paulo C. [2] ; Braga, Gilberto U. L. [3] ; Rangel, Drauzio E. N. [4]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, EEL, BR-12602810 Lorena, SP - Brazil
[2] Univ Vale Paraiba, BR-12244000 Sao Jose Dos Campos, SP - Brazil
[3] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
[4] Univ Fed Goias, Inst Patol Trop & Saude Publ, BR-74605050 Goiania, Go - Brazil
Total Affiliations: 4
Document type: Journal article
Source: FUNGAL BIOLOGY; v. 122, n. 6, SI, p. 592-601, JUN 2018.
Web of Science Citations: 5
Abstract

The low survival of insect-pathogenic fungi when used for insect control in agriculture is mainly due to the deleterious effects of ultraviolet radiation and heat from solar irradiation. In this study, conidia of 15 species of entomopathogenic fungi were exposed to simulated full-spectrum solar radiation emitted by a Xenon Test Chamber Q-SUN (R) XE-3-HC 340S (Q-LAB Corporation, Westlake, OH, USA), which very closely simulates full-spectrum solar radiation. A dendrogram obtained from cluster analyses, based on lethal time 50 % and 90 % calculated by Probit analyses, separated the fungi into three clusters: cluster 3 contains species with highest tolerance to simulated full-spectrum solar radiation, included Metarhizium acridum, Cladosporium herbarum, and Trichothecium roseum with LT50 > 200 min irradiation. Cluster 2 contains eight species with moderate UV tolerance: Aschersonia aleyrodis, Isaria fumosorosea, Mariannaea pruinosa, Metarhizium anisopliae, Metarhizium brunneum, Metarhizium robertsii, Simplicillium Ianosoniveum, and Torrubiella homopterorum with LT50 between 120 and 150 min irradiation. The four species in cluster 1 had the lowest UV tolerance: Lecanicillium aphanocladii, Beauveria bassiana, Tolypocladium cylindrosporum, and Tolypocladium inflatum with LT50 < 120 min irradiation. The QSUN Xenon Test Chamber XE3 is often used by the pharmaceutical and automotive industry to test light stability and weathering, respectively, but it was never used to evaluate fungal tolerance to full-spectrum solar radiation before. We conclude that the equipment provided an excellent tool for testing realistic tolerances of fungi to full-spectrum solar radiation of microbial agents for insect biological control in agriculture. (C) 2018 British Mycological Society. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 12/15204-8 - Mechanistic study of the photodynamic inactivation of human and plant pathogenic fungi
Grantee:Gilberto Úbida Leite Braga
Support type: Regular Research Grants
FAPESP's process: 13/50518-6 - Stress related genes are induced by visible light during mycelial growth resulting in increased conidial tolerance to stress conditions
Grantee:Drauzio Eduardo Naretto Rangel
Support type: Regular Research Grants
FAPESP's process: 13/25964-2 - Visible light during mycelial growth alters gene expression and improves stress tolerance in entomopathogenic fungi
Grantee:Luciana Pereira Dias
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 10/06374-1 - Visible light during growth enhances conidial tolerance to different stress conditions in fungi
Grantee:Drauzio Eduardo Naretto Rangel
Support type: Research Grants - Young Investigators Grants