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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Detection of HTLV-1 proviral DNA in BM mononuclear cells and cultured mesenchymal stromal cells isolated from patients with HTLV-1 infection

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Rodrigues, Evandra Strazza [1, 2] ; Favarin, Maria do Carmo [2] ; de Macedo, Mayra Dorigan [1, 2] ; Otaguiri, Katia Kaori [1, 2] ; Orellana, Maristela Delgado [3, 2] ; Takayanagui, Osvaldo Massaiti [3] ; Bonini Palma, Patricia Vianna [2] ; Slavov, Svetoslav Nanev [3, 2] ; Covas, Dimas Tadeu [3, 2] ; Kashima, Simone [1, 2]
Total Authors: 10
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Toxicol & Bromatol Anal, Sao Paulo - Brazil
[2] Univ Sao Paulo, Reg Blood Ctr Ribeirao Preto, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Internal Med, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: VIROLOGY; v. 519, p. 145-155, JUN 2018.
Web of Science Citations: 0

The bone marrow (BM) biology during HTLV-1 infection is obscure. In this study, we investigated BM mononuclear cells and mesenchymal stromal cells (MSC) from HTLV-1 asymptomatic and symptomatic individuals. An infiltration of CD4(+)T-cell lymphocytes in the BM of HTLV-1-infected individuals was observed when compared to healthy controls. The provirus detection in the BM CD4(+)T cells confirmed the presence of integrated HTLV DNA. In regard to MSC, we observed that the number of fibroblast progenitor cells was lower in HTLV-1 infected individuals than in healthy controls. Isolated HTLV-1 infected BM-MSC demonstrated surface expression markers and in vitro differentiation potential similar to uninfected individuals. The presence of HTLV-1 proviral DNA in the BM-MSC of HTLV-1-infected patients was demonstrated but no p19 antigen was detected in supernatant from cultured MSC. We suppose that HTLV-1 infects human MSC probably by cell-to-cell contact from the infected CD4(+)T-lymphocytes infiltrated into the bone marrow. (AU)

FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
Grantee:Dimas Tadeu Covas
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC