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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Crotoxin Isolated from Crotalus durissus terrificus Venom Modulates the Functional Activity of Dendritic Cells via Formyl Peptide Receptors

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Author(s):
Freitas, A. P. [1, 2] ; Favoretto, B. C. [2] ; Clissa, P. B. [2] ; Sampaio, S. C. [3, 4] ; Faquim-Mauro, E. L. [1, 2]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Inst Biomed Sci, Dept Immunol, Sao Paulo, SP - Brazil
[2] Butantan Inst, Immunopathol Lab, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo, SP - Brazil
[4] Butantan Inst, Physiopathol Lab, Sao Paulo, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF IMMUNOLOGY RESEARCH; 2018.
Web of Science Citations: 3
Abstract

The Crotalus durissus terrificus rattlesnake venom, its main toxin, crotoxin (CTX), and its crotapotin (CA) and phospholipase A(2) (CB) subunits modulate the immune system. Formyl peptide receptors (FPRs) and lipoxin A(4) (LXA(4)) are involved in CTX's effect on macrophages and neutrophils. Dendritic cells (DCs) are plasticity cells involved in the induction of adaptive immunity and tolerance maintenance. Therefore, we evaluated the effect of CTX, CA or CB on the maturation of DCs derived from murine bone marrow (BM). According to data, CTX and CB-but not CA-induced an increase of MHC-II, but not costimulatory molecules on DCs. Furthermore, CTX and CB inhibited the expression of costimulatory and MHC-II molecules, secretion of proinflammatory cytokines and NF-kappa Bp65 and p38/ERK1/2-MAPK signaling pathways by LPS-incubated DCs. Differently, CTX and CB induced IL-10, PGE(2) and LXA(4) secretion in LPS-incubated DCs. Lower proliferation and IL-2 secretion were verified in coculture of CD3(+) cells and DCs incubated with LPS plus CTX or CB compared with LPS-incubated DCs. The effect of CTX and CB on DCs was abolished in cultures incubated with a FPRs antagonist. Hence, CTX and CB exert a modulation on functional activity of DCs; we also checked the involvement the FPR family on cell activities. (AU)

FAPESP's process: 11/23735-0 - Mechanisms involved in the modulation of the immune response induced by high molecular weight components of Ascaris suum extract and crotoxin isolated from Crotalus durissus terrificus venom
Grantee:Eliana Faquim de Lima Mauro
Support type: Regular Research Grants
FAPESP's process: 10/10393-1 - Study of the involvement of DC-SIGN and MR receptors in the mechanisms of immunesuppression induced by high molecular weight components of Ascaris suum extract
Grantee:Bruna Cristina Favoretto
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/13085-7 - Molecular mechanisms involved in the suppression of the immune response to helminths: intracellular signaling pathways induced by the interaction of Ascaris suum antigens with DC-SIGN and MR on dendritic cells
Grantee:Bruna Cristina Favoretto
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 15/15608-0 - Evaluation of the molecular mechanisms involved in the modulation of immune response by high molecular weight components of Ascaris suum, crotoxin and Phospholipase A2 from Crotalus durissus terrificus venom
Grantee:Eliana Faquim de Lima Mauro
Support type: Regular Research Grants