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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vivo investigation of the volatile metabolome of antiphytopathogenic yeast strains active against Penicillium digitatum using comprehensive two-dimensional gas chromatography and multivariate data analysis

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Belinato de Souza, Joao Raul [1, 2] ; Kupper, Katia Cristina [3] ; Augusto, Fabio [1, 2]
Total Authors: 3
[1] Univ Estadual Campinas, UNICAMP, Inst Chem, CP 6154, BR-13083970 Campinas, SP - Brazil
[2] Natl Inst Bioanalyt Sci & Technol INCTBio, CP 6154, BR-13083970 Campinas, SP - Brazil
[3] Sylvio Moreira Citrus Res Ctr, Campinas Agron Inst, Cordeiropolis, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Microchemical Journal; v. 141, p. 204-209, SEP 2018.
Web of Science Citations: 3

Penicillium digitatum is the responsible for green mold decay on citrus fruits, which contributes to economic losses in agriculture industry during storage, transportation and in the market. Biocontrol agents may be a viable and low-cost alternative in the control of different plant pathogens: metabolites from some yeast strains can prevent infection, decreasing host tissue colonization and reducing pathogen survival and sporulation with varying degrees of efficiency. Eight S. cerevisae strains and one Candida stellimalicola strain all were evaluated in vitro as biological control agents against P. digitatum. After in vitro tests the headspace of yeast strains were extracted in vivo using an optimized HS-SPME + GC x GC-MS method. The correlation between antiphytopathogenic activity with the volatile metabolite profiles was assessed using principal component analysis. Nine compounds were associated with the volatile metabolome of strains with higher antifungal action: ethyl propionate, 3-methyl-1-butanol, 2-methyl-1-butanol, ethyl butyrate, 3-methylbutyl acetate, 3-methylbutyl propionate, n-pentyl butyrate, phenylethyl alcohol and 2-phenethyl acetate. This study allowed for the first time a comprehensive overview of the effect of individual volatile metabolites from yeast strains on P. digitatum infection. (AU)

FAPESP's process: 16/20547-2 - Characterization of the metabolome of anti-phytopatogen microorganisms by Multidimensional Gas Chromatography and Simple / Tandem Mass Spectrometry (GC×GC-QMS and GC×GC-MS/MS)
Grantee:Joao Raul Belinato de Souza
Support type: Scholarships in Brazil - Doctorate