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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification of Leishmania (Viannia) species and clinical isolates of Leishmania (Leishmania) amazonensis from Brazil using PCR-RFLP of the heat-shock protein 70 gene reveals some unexpected observations

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Espada, Caroline R. [1] ; Ortiz, Paola A. [1] ; Shaw, Jeffrey J. [1] ; Barral, Aldina M. P. [2] ; Costa, Jackson M. L. [2] ; Uliana, Silvia R. B. [1] ; Coelho, Adriano C. [3, 1]
Total Authors: 7
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508900 Sao Paulo, SP - Brazil
[2] Fundacao Oswaldo Cruz FIOCRUZ, Inst Goncola Moniz, BR-40296710 Salvador, BA - Brazil
[3] Univ Estadual Campinas, Inst Biol, Dept Biol Anim, BR-13083862 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Web of Science Citations: 7

Hsp70 is a cytoplasmic heat-shock protein, encoded by a multicopy tandemly repeated gene that has recently been gaining popularity as a valuable marker for typing Leish mania species. In this study, we used a previously described hsp70 PCR-RFLP method for identifying Brazilian Leishmania isolates. We identified two distinct L (L) amazonensis hsp70 alleles that resulted in two different RFLP patterns. Also, we found RFLP polymorphisms amongst L (Viannia) naiffi strains. The profiles of both L (V.) shawi and L (V.) lindenbergi were very similar to those of other L (Viannia) species. The observations described herein reflect the polymorphism found within species of Leishmania and indicate that results from this hsp70 PCR-RFLP method should be used with caution when typing isolates from clinical cases of leishmaniasis and Leishmania species from Brazil. (C) 2018 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 16/23405-4 - Susceptibility to miltefosine in Leishmania (Viannia) braziliensis clinical isolates: phenotypic characterization and investigation of the basis involved in reduced susceptibility to the drug
Grantee:Caroline Ricce Espada
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 11/20484-7 - Tamoxifen in the treatment of leishmaniasis: evaluation of efficacy in combination therapy schemes and study of the antileishmanial mechanism of action
Grantee:Silvia Reni Bortolin Uliana
Support type: Regular Research Grants
FAPESP's process: 12/14629-5 - Mechanisms of action and resistance to miltefosine in Leishmania spp.
Grantee:Adriano Cappellazzo Coelho
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 15/09080-2 - Evaluation of candidate drugs for the treatment of Leishmaniasis in Brazil
Grantee:Silvia Reni Bortolin Uliana
Support type: Regular Research Grants