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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cytotoxicity Assessment of a Poly(methyl methacrylate) Synthesized for the Direct Fabrication of Bone Tissues

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Linan, Lamia Zuniga [1] ; Nascimento Lima, Nadson Murilo [1] ; Benatti, Carol [1] ; Xavier, Mariana [1] ; Rodrigues, Ana A. [1] ; Manenti, Flavio [2] ; Jardini, Andre [1] ; Maciel Filho, Rubens [1] ; Gilioli, Rovilson [3]
Total Authors: 9
[1] Univ Estadual Campinas, Dept Desenvolvimento Proc & Prod, Campinas, SP - Brazil
[2] Politecn Milan, Dipartamento Chim Mat & Engn Chim Giulio Natta, Milan, Lombardia - Italy
[3] Univ Estadual Campinas, Ctr Multidisciplinar Invest Biol Area Ciencia Ani, Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Brazilian Archives of Biology and Technology; v. 61, 2018.
Web of Science Citations: 0

A cytotoxicity study is performed on a poly(methyl methacrylate) polymer (PMMA) to be used for the fabrication of bone tissue by Rapid Prototyping (RP). The solution polymerization is conducted in a pilot plant reactor using more appropriated reagents in consideration of the medical application. Moreover, the polymer is efficiently handled to avoid the side effect of the monomer, reducing the concentration of this specie to 287,731 mu g MMA/kg PMMA. The cytotoxicity of the polymer is determined through growth monitoring, adherence and morphology of L-929 cells. Additionally, MTT and LIVE/DEAD tests are performed. The results showed continuous and progressive growth of the cells on the surface of the specimens. Moreover, the material did not influence on the viability of mesenchymal cells and inverted fluorescence microscopy images showed a polyanionic dye calcein well retained in the cells in contact with the PMMA as well as the negative control after 72 hours. Thus, the polymer was efficiently synthesized and handled for the expected demands. (AU)

FAPESP's process: 10/16952-2 - Modeling and optimal control by fuzzy logic for processes of production of biomaterials
Grantee:Nádson Murilo Nascimento Lima
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 11/09631-8 - Study and modeling of poly(methyl methacrylate) production process to obtain artificial bone tissue
Grantee:Rubens Maciel Filho
Support type: Regular Research Grants
FAPESP's process: 09/09092-0 - Study and modeling of the process of production of PMMA for the fabrication of artificial bone cement
Grantee:Lamia Zuniga Linan
Support type: Scholarships in Brazil - Post-Doctorate