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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antineoplastic Effects of NF-kappa B Inhibition by DHMEQ (Dehydroxymethylepoxyquinomicin) Alone and in Co-treatment with Radio-and Chemotherapy in Medulloblastoma Cell Lines

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Author(s):
Ramos, Priscila M. M. [1] ; Pezuk, Julia A. [1, 2] ; Castro-Gamero, Angel M. [1, 3] ; Oliveira, Harley F. [4] ; Scrideli, Carlos A. [5] ; Umezawa, Kazuo [6] ; Tone, Luiz G. [5, 1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Ribeirao Preto, SP - Brazil
[2] Univ Anhaguera Sao Paulo, UNIAN SP, Sao Paulo, SP - Brazil
[3] Univ Fed Alfenas, Inst Nat Sci, Alfenas, MG - Brazil
[4] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Clin Med, Ribeirao Preto, SP - Brazil
[5] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pediat, Ribeirao Preto, SP - Brazil
[6] Aichi Med Univ, Dept Mol Target Med Screening, Nagakute, Aichi - Japan
Total Affiliations: 6
Document type: Journal article
Source: ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY; v. 18, n. 4, p. 541-549, 2018.
Web of Science Citations: 2
Abstract

Background: NF-kappa B is a transcription factor involved in the transcriptional regulation of a large number of genes related to tumorigenesis in several cancer cell types, and its inhibition has been related to anticancer effect. DHMEQ (Dehydroxymethylepoxyquinomicin) is a compound that blocks the translocation of NF-kappa B from the cytoplasm to the nucleus, thus inhibiting its activity as a transcriptional activator. Several studies have shown the antineoplastic effects of DHMEQ in numerous tumor types, however, there are no surveys that tested their effects in MB. Objectives: The aim of the present study was to evaluate the effects of DHMEQ as NF-kappa B inhibitor in pediatric MB cell lines. Method: We used the UW402, UW473 and ONS-76 medulloblastoma (MB) cell lines to verify the effect of DHMEQ on proliferation, clonogenic capacity, apoptosis, cell invasion and migration, and evaluated the effect of the combination with other drugs and the potential as a radiosensitizator. Results: A significant decrease in the cell growth, a strong inhibition of the clonogenic capacity, migration and cell invasion was observed after NF-kappa B inhibition in the three MB cell lines. Conversely, increased level of apoptosis rates were demonstrated. Additionally, treatments with DHMEQ combined with other chemotherapeutic agents were synergic in most points, and a strong radiosensitization by this compound was observed in the three MB cell lines. Conclusion: DHMEQ has potential antitumor effect on MB cells, and it may be considered a new therapeutic agent to improve treatment approaches in MB. (AU)

FAPESP's process: 11/01026-8 - POLO-LIKE kinase in medulloblastoma: PLKs genes expression evaluation and in vitro effects of pharmacological inhibition of PLK1
Grantee:Julia Alejandra Pezuk
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 10/18792-2 - Evaluation of anti-neoplastic effects of inhibition of NFkB by DHMEQ (dehydroxymethylepoxyquinomicin) in medulloblastoma cell lines
Grantee:Priscila Maria Manzini Ramos
Support type: Scholarships in Brazil - Doctorate