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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Insulin-Like Growth Factor-I as an Effector Element of the Cytokine IL-4 in the Development of a Leishmania major Infection

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Author(s):
Reis, Luiza C. [1] ; Ramos-Sanchez, Eduardo Milton [1] ; Petitto-Assis, Fabricio [1] ; Nerland, Audun H. [2] ; Hernandez-Valladares, Maria [3] ; Selheim, Frode [3] ; Floeter-Winter, Lucile Maria [4] ; Goto, Hiro [5, 1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, IMTSP, Sao Paulo, SP - Brazil
[2] Univ Bergen, Dept Clin Sci, Bergen - Norway
[3] Univ Bergen, Dept Biomed, Bergen - Norway
[4] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Sao Paulo, SP - Brazil
[5] Univ Sao Paulo, Fac Med, Dept Med Prevent, Sao Paulo, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Mediators of Inflammation; 2018.
Web of Science Citations: 1
Abstract

Certain cytokines modulate the expression of insulin-like growth factor-(IGF-) I. Since IL-4 and IGF-I promote growth of the protozoan Leishmania major, we here addressed their interaction in downregulating the expression of Igf-I mRNA using small interfering RNA (siRNA) in Leishmania major-infected macrophages. Parasitism was decreased in the siRNA-treated cells compared with the nontreated cells, reversed by the addition of recombinant IGF-I (rIGF-I). In IL-4-stimulated macrophages, parasitism and the Igf-I mRNA amount were increased, and the effects were nullified upon siRNA transfection. IGF-I downregulation inhibited both parasite and macrophage arginase activation even in IL-4-stimulated cells. Searching for intracellular signaling components shared by IL-4 and IGF-I, upon siRNA transfection, phosphorylated p44, p38, and Akt proteins were decreased, affecting the phosphatidylinositol-3-kinase (PI3K)/Akt pathway. In L. major-infected C57BL6-resistant mice, the preincubation of the parasite with rIGF-I changed the infection profile to be similar to that of susceptible mice. We conclude that IGF-I constitutes an effector element of IL-4 involving the PI3K/Akt pathway during L. major infection. (AU)

FAPESP's process: 08/04106-0 - The role of insulin like-growth factor I (IGF-I) in the modulation of cytokines effect in resistance and susceptibility in murine cutaneous leishmaniasis infected by Leishmania (L.) major
Grantee:Luiza de Campos Reis
Support type: Scholarships in Brazil - Doctorate