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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Detrimental effects detected in exfoliated buccal cells from anesthesiology medical residents occupationally exposed to inhalation anesthetics: An observational study

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Braz, Mariana G. [1] ; Souza, Katina M. [1] ; Lucio, Lorena M. C. [1] ; Di Renzo, Giulia C. C. [1] ; Feliciano, Luciana M. [2] ; Marcondes, Joao Paulo C. [2] ; Oliver Chen, C. -Y. [3] ; Braz, Jose Reinaldo C. [1] ; Braz, Leandro G. [1]
Total Authors: 9
[1] Univ Estadual Paulista UNESP, Dept Anesthesiol, Fac Med, Botucatu, SP - Brazil
[2] Univ Estadual Paulista UNESP, Inst Biociencias, Dept Genet, Botucatu, SP - Brazil
[3] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 - USA
Total Affiliations: 3
Document type: Journal article
Web of Science Citations: 1

Operating room professionals are scarcely aware of their individual occupational exposure to waste anesthetic gases (WAGs). Medical residents spend several hours per day in operating rooms and consequently experience occupational exposure to WAGs. Considering that no studies have yet evaluated the potential toxicity in medical residents exposed to WAGs using the buccal micronucleus cytome (BMCyt) assay, this pioneering study aimed to compare the BMCyt assay markers, including DNA damage, cell proliferation, and cell death in the exfoliated buccal cells of surgery and anesthesiology residents occupationally exposed to WAGs. The study enrolled a total of 60 physicians, including internal medicine residents (unexposed group), and residents from surgery and anesthesiology programs who were occupationally exposed to sevoflurane, isoflurane and nitrous oxide. WAGs were measured, and the mean values were higher than the international recommendation. The anesthesiology residents (high exposure) showed statistically significant lower frequencies of basal cells, and statistically significant higher frequencies of micronuclei, karyorrhexis, pyknosis, and differentiated cells than did the unexposed group; karyolysis frequencies were significantly higher in anesthesiology residents than were those in the unexposed group or in surgical residents (low exposure). The findings suggest a genetic risk for young professionals exposed to WAGs at the beginning of their careers. Thus, exposure to high WAGs concentrations leads to impairment of the buccal cell proliferative potential, genomic instability and cell death, especially in anesthesiology residents, demonstrating an early impact on their health. (AU)

FAPESP's process: 13/21130-0 - Correlation between concentration of waste anesthetic gases with oxidative stress and immune response in medical residents occupationally exposed
Grantee:Mariana Gobbo Braz
Support type: Regular Research Grants
FAPESP's process: 13/05084-8 - Evaluation of DNA damage in Anesthesiologists
Grantee:Leandro Gobbo Braz
Support type: Regular Research Grants