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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

HU-Lacking Mutants of Salmonella enterica Enteritidis Are Highly Attenuated and Can Induce Protection in Murine Model of Infection

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Milanez, Guilherme P. [1] ; Werle, Catierine H. [1] ; Amorim, Mariene R. [1] ; Ribeiro, Rafael A. [1] ; Tibo, Luiz H. S. [1] ; Roque-Barreira, Maria Cristina [2] ; Oliveira, Aline F. [2] ; Brocchi, Marcelo [1]
Total Authors: 8
[1] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut Microbiol & Immunol, Campinas, SP - Brazil
[2] Univ Sao Paulo, Dept Cellular & Mol Biol, Fac Med Ribeirao Preto, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Web of Science Citations: 0

Salmonella enterica infection is a major public health concern worldwide, particularly when associated with other medical conditions. The serovars Typhimurium and Enteritidis are frequently associated with an invasive illness that primarily affects immunocompromised adults and children with HIV, malaria, or malnutrition. These serovars can also cause infections in a variety of animal hosts, and they are the most common isolates in poultry materials. Here, we described S. Enteritidis mutants, where hupA and hupB genes were deleted, and evaluated their potential use as liveattenuated vaccine candidates. In vitro, the mutants behaved like S. Typhimurium described previously, but there were some particularities in macrophage invasion and survival experiments. The virulence and immunogenicity of the mutant lacking both hupA and hupB (PT4 Delta hupAB) were evaluated in a BALB/c mice model. This mutant was highly attenuated and could, therefore, be administrated at doses higher than 10(9) CFU/treatment, which was sufficient to protect all treated mice challenged with the wild-type parental strain with a single dose. Additionally, the PT4 Delta hupAB strain induced production of specific IgG and IgA antibodies against Salmonella and TH1-related cytokines (IFN-gamma and TNE-alpha), indicating that this strain can induce systemic and mucosal protection in the murine model. Additional studies are needed to better understand the mechanisms that lead to attenuation of the double-mutant PT4 Delta hupAB and to elucidate the immune response induced by immunization using this strain. However, our data allow us to state that hupAB mutants could be potential candidates to be explore as live-attenuated vaccines. (AU)

FAPESP's process: 13/04088-0 - Lectin from pathogens
Grantee:Maria Cristina Roque Antunes Barreira
Support type: Research Projects - Thematic Grants
FAPESP's process: 12/05382-6 - Virulence evaluation and Differential gene expression in vivo of Salmonella enterica mutants for nucleoid associated proteins
Grantee:Guilherme Paier Milanez
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/13412-8 - Salmonella enterica: roles of NAPS in pathogenicity and virulence
Grantee:Marcelo Brocchi
Support type: Regular Research Grants
FAPESP's process: 14/05359-0 - Distribution of paracoccin on the surface of Paracoccidioides brasiliensis and interaction with innate immunity cell receptors: role in the acute phase of paracoccidioidomycosis
Grantee:Aline Ferreira de Oliveira Pereira
Support type: Scholarships in Brazil - Post-Doctorate