Low dose of chlorine exposure exacerbates nasal and pulmonary allergic inflammation in mice

Author(s):Show less -

de Genaro, Isabella Santos ^{[1, 2]} ; de Almeida, Francine Maria ^[1] ; Hizume-Kunzler, Deborah Camargo ^{[1, 3]} ; Moriya, Henrique Takachi ^[4] ; Silva, Ronaldo Aparecido ^[1] ; Goncalves Cruz, Joao Carlos ^[5] ; Lopes, Renan Boeira ^[5] ; Righetti, Renato Fraga ^{[6, 1]} ; Vieira, Rodolfo de Paula ^{[7, 8]} ; Saiki, Mitiko ^[9] ; Martins, Milton Arruda ^[1] ; Lopes Calvo Tiberio, Iolanda de Fatima ^[1] ; Arantes-Costa, Fernanda Magalhaes ^[1] ; Saraiva-Romanholo, Beatriz Mangueira ^{[5, 1, 2]}

Affiliation

^[1] Univ Sao Paulo, Sch Med, Dept Med LIM 20, Sao Paulo - Brazil

^[2] Publ Employee Sao Paulo Hosp IAMSPE, Sao Paulo - Brazil

^[3] Univ Estado Santa Catarina, Dept Phys Therapy LaPEx, Florianopolis, SC - Brazil

^[4] Univ Sao Paulo, Escola Politecn, Biomed Engn Lab, Sao Paulo - Brazil

^[5] Univ City Sao Paulo UNICID, Sao Paulo - Brazil

^[6] Sirio Libanes Hosp, Sao Paulo - Brazil

^[7] Univ Brasil, Postgrad Program Bioengn, Sao Paulo - Brazil

^[8] Brazilian Inst Teaching & Res Pulm & Exercise Imm, Sao Jose Dos Campos - Brazil

^[9] IPEN CNEN SP, Nucl & Energy Res Inst, Sao Paulo - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 8, AUG 22 2018.
• Web of Science Citations: 2

Abstract

Work-exacerbated asthma (WEA) is defined as preexisting asthma that worsens with exposure to irritants {[}e.g., chlorine (Cl-2) derivatives] in the workplace. The maximum allowable concentration in the workplace of Cl-2 exposure is 3 mg/m(3) (described in OSHA). We investigated in an experimental asthma model in mice the effects of a single exposure to a sodium hypochlorite dose with this allowed chlorine concentration and a tenfold higher dose. Acute chlorine exposure at 3.3 mg/m(3) in the OVA-sensitized group increased eosinophils in the peribronquial infiltrate, cytokine production, nasal mucus production and the number of iNOS positive cells in the distal lung compared to only sensitized mice. The exposure to a higher dose of 33.3 mg/m(3) in the OVA-sensitized group resulted in an increase in respiratory system elastance, in the total and differential numbers of inflammatory cells in bronchoalveolar lavage fluid, IL-4, IL-5, and IL-17 in the lungs, eosinophils in peribronquial infiltrate and mucus content in nasal compared to non-exposed and sensitized animals. In this asthma model, chorine exposures at an allowable dose, contributed to the potentiation of Th2 responses. The functional alterations were associated with increased iNOS and ROCK-2 activation in the distal lung. (AU)

• FAPESP's process: 09/53904-9 - Effects of environment and lifestyle on asthma and chronic obstructive pulmonary disease: studies with experimental animals and people with asthma
• Grantee: Milton de Arruda Martins
• Support type: Research Projects - Thematic Grants
• FAPESP's process: 12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals
• Grantee: Rodolfo de Paula Vieira
• Support type: Research Grants - Young Investigators Grants