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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Metabolic gene expression and epigenetic effects of the ketone body beta-hydroxybutyrate

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Sangalli, Juliano Rodrigues [1] ; Sampaio, Rafael Vilar [1] ; del Collado, Maite [1] ; da Silveira, Juliano Coelho [1] ; Camara De Bem, Tiago Henrique [1] ; Perecin, Felipe [1] ; Smith, Lawrence Charles [2] ; Meirelles, Flavio Vieira [1]
Total Authors: 8
[1] Univ Sao Paulo, Dept Vet Med, Fac Anim Sci & Food Engn, BR-13635900 Pirassununga, SP - Brazil
[2] Univ Montreal, Fac Med Vet, Ctr Rech Reprod & Fertil, St Hyacinthe, PQ H3T 1J4 - Canada
Total Affiliations: 2
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 8, SEP 13 2018.
Web of Science Citations: 2

The rapid decline in fertility that has been occurring to high-producing dairy cows in the past 50 years seems to be associated with metabolic disturbances such as ketosis, supporting the need for research to improve our understanding of the relations among the diet, metabolism and embryonic development. Recently, the ketone body beta-hydroxybutyrate (BOHB) was demonstrated to be a potent inhibitor of histone deacetylases (HDACs). Herein, we performed a series of experiments aiming to investigate the epigenetic effects of BOHB on histone acetylation in somatic cells, cumulus-oocyte complexes (COCs) and somatic cell nuclear transfer (SCNT) embryos. Treatment with BOHB does not increase histone acetylation in cells but stimulates genes associated with ketolysis and master regulators of metabolism. We further demonstrated that maturing COCs with high levels of BOHB does not affect their maturation rate or histone acetylation but increases the expression of PPARA in cumulus cells. Treatment of somatic cell nuclear transfer zygotes with BOHB causes hyperacetylation, which is maintained until the blastocyst stage, causing enhanced FOXO3A expression and blastocyst production. Our data shed light on the epigenetic mechanisms caused by BOHB in bovine cells and embryos and provide a better understanding of the connection between nutrition and reproduction. (AU)

FAPESP's process: 14/22887-0 - Cell-secreted vesicles containing miRNAs modulate epigenetic changes during in vitro culture of bovine gametes and embryos
Grantee:Juliano Coelho da Silveira
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 16/13416-9 - Use of the ketone body beta-hydroxybutyric acid as an experimental tool to study the link among metabolism, epigenetic and reproduction in cattle
Grantee:Juliano Rodrigues Sangalli
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/21097-5 - Dietary effects on the sperm epigenome
Grantee:Juliano Rodrigues Sangalli
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 12/50533-2 - GIFT: genomic improvement of fertilization traits in Danish and Brazilian cattle
Grantee:Marcelo Fábio Gouveia Nogueira
Support type: Research Projects - Thematic Grants
FAPESP's process: 13/06673-7 - Use of histones deacetylases inhibitors in bovine somatic cell nuclear transfer.
Grantee:Juliano Rodrigues Sangalli
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/21034-3 - Intra and intercellular routes during foliculogenesis and in vivo and in vitro oocyte maturation
Grantee:Felipe Perecin
Support type: Regular Research Grants
FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
Grantee:Dimas Tadeu Covas
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC