Treatment with cyclic adenosine monophosphate modulators prior to in vitro maturation alters the lipid composition and transcript profile of bovine cumulus-oocyte complexes and blastocysts

Author(s):Show less -

Razza, Eduardo M. ^[1] ; Sudano, Mateus J. ^[2] ; Fontes, Patricia K. ^[3] ; Franchi, Fernanda F. ^[3] ; Belaz, Katia Roberta A. ^[4] ; Santos, Priscila H. ^[3] ; Castilho, Anthony C. S. ^{[5, 6]} ; Rocha, Daniele F. O. ^[4] ; Eberlin, Marcos N. ^[4] ; Machado, Mariana F. ^[3] ; Nogueira, Marcelo F. G. ^{[3, 6]}

Affiliation

^[1] Nove de Julho Univ, Rua Nicolau Assis 15, BR-17011102 Bauru, SP - Brazil

^[2] Fed Univ Pampa, Sch Vet Med, BR 472 Km 592, Caixa Postal 118, BR-97508000 Uruguaiana, RS - Brazil

^[3] Univ Sao Paulo State, Inst Biosci, Dept Pharmacol, Dist Rubiao Jr S-N, BR-18618970 Botucatu, SP - Brazil

^[4] Univ Estadual Campinas, Inst Chem, ThoMSon Mass Spectrometry Lab, Rua Sergio Buarque de Holanda S-N, BR-13083859 Campinas, SP - Brazil

^[5] Univ Western Sao Paulo, Rodovia Raposo Tavares, Km 572 Bairro Limoeiro, BR-19067175 Presidente Prudente, SP - Brazil

^[6] Univ Sao Paulo State, Fac Sci & Letters, Dept Biol Sci, Ave Dom Antonio 2100, BR-19806900 Assis, SP - Brazil

• Document type: Journal article
• Source: REPRODUCTION FERTILITY AND DEVELOPMENT; v. 30, n. 10, p. 1314-1328, SEP 2018.
• Web of Science Citations: 2

Abstract

Mammalian oocytes resume meiosis spontaneously after removal from the ovarian follicle. We tested the effects of a 2-h prematuration treatment (Pre-IVM) with forskolin (FSK) and 3-isobutyl-1-methylxanthine (IBMX) in bovine cumulus-oocyte complexes (COCs) on the lipid content of oocytes and blastocysts, on the membrane lipid composition of blastocysts and on the transcriptional profiling of cumulus cells and blastocysts in a high-throughput platform. Embryonic development rates to the morula (mean 56.1%) or blastocyst (mean 26.3%) stages were unaffected by treatment. Lipid content was not affected after Pre-IVM, but was increased after IVM in treated oocytes. Conversely, the lipid content was reduced in Pre-IVM blastocysts. Pre-IVM COCs generated blastocysts containing blastomeres with more unsaturated lipids in their membranes. Pre-IVM also altered the relative abundance of 31 gene transcripts after 2 h and 16 transcripts after 24 h in cumulus cells, while seven transcripts were altered in blastocysts. Our results suggest that the Pre-IVM treatment affected the lipid composition and transcriptional profiles of COCs and blastocysts. Therefore, Pre-IVM with FSK and IBMX could be used either to prevent spontaneous meiotic resumption during IVM or to modulate lipid composition in the membrane and cytoplasm of blastocysts, potentially improving bovine embryos. (AU)

• FAPESP's process: 13/05083-1 - Modulation of oocyte nuclear maturation and its effects on cumulus-oocyte complexes and bovine embryo production in vitro
• Grantee: Marcelo Fábio Gouveia Nogueira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/23409-9 - Differential gene expression, ultrastructural analysis and evaluation of the lipid profile of in vitro produced bovine blastocysts from oocytes matured conventionally or via SPOM system
• Grantee: Eduardo Montanari Razza
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/50533-2 - GIFT: genomic improvement of fertilization traits in Danish and Brazilian cattle
• Grantee: Marcelo Fábio Gouveia Nogueira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 12/10737-8 - COMPARISON BETWEEN THE CONVENTIONAL IN VITRO MATURATION AND THE SIMULATION OF OOCYTE PHYSIOLOGICAL MATURATION (SPOM) IN BOVINE CUMULUS-OOCYTE COMPLEX
• Grantee: Mariana Fernandes Machado
• Support Opportunities: Scholarships in Brazil - Post-Doctoral