Integrated diversity analysis of the microbial community in a reverse osmosis system from a Brazilian oil refinery

Author(s):Show less -

Belgini, Daiane R. B. ^{[1, 2]} ; Siqueira, Virginia M. ^[1] ; Oliveira, Douglas M. ^[1] ; Fonseca, Samantha G. ^[1] ; Piccin-Santos, Viviane ^[1] ; Dias, Roberto S. ^[3] ; Quartaroli, Larissa ^[4] ; Souza, Rodrigo S. ^[4] ; Torres, Ana Paula R. ^[5] ; Sousa, Maira P. ^[5] ; Silva, Claudio M. ^[4] ; Silva, Cynthia C. ^[6] ; De Paula, Sergio O. ^[3] ; Oliveira, Valeria M. ^[2]

Affiliation

^[1] Campinas Univ UNICAMP, Res Ctr Chem Biol & Agr CPQBA, Microbial Resources Div, POB 6171, BR-13081970 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Inst Biol, UNICAMP, POB 6109, BR-13083970 Campinas, SP - Brazil

^[3] Fed Univ Vicosa UFV, Gen Biol Dept, BR-36570000 Vicosa, MG - Brazil

^[4] Fed Univ Vicosa UFV, Bldg Engn Dept, BR-36570000 Vicosa, MG - Brazil

^[5] PETROBRAS Res & Dev Ctr CENPES, Biotechnol Management, Av Horatio Macedo 950, Ala C, BR-21941915 Rio De Janeiro, RJ - Brazil

^[6] Fed Univ Vicosa UFV, Microbiol Dept, BR-36570000 Vicosa, MG - Brazil

• Document type: Journal article
• Source: Systematic and Applied Microbiology; v. 41, n. 5, p. 473-486, SEP 2018.
• Web of Science Citations: 5

Abstract

Oil refineries are known for the large volume of water used in their processes, as well as the amount of wastewater generated at the end of the production chain. Due to strict environmental regulations, the recycling of water has now become a viable alternative for refineries. Among the many methods available to treat wastewater for reuse, the use of membranes in reverse osmosis systems stands out due to several economic and environmental benefits. However, these systems are vulnerable to contamination and deposition of microorganisms, mainly because of the feedwater quality. In this study, the microbial diversity of feedwater and reverse osmosis membranes was investigated using a combination of culture-dependent and culture-independent methods in order to characterize the microorganisms colonizing and deteriorating the membranes. In total, 37 bacterial isolates, 17 filamentous fungi and approximately 400 clones were obtained and analyzed. Among the bacterial genera identified, the most represented were Sphingobium, Acidovorax, Microbacterium, Rhizobium and Shinella. The results revealed genera that acted as candidate key players in initial biofilm formation in membrane systems, and provided important information concerning the microbial ecology of oligotrophic aquatic systems. (C) 2018 Elsevier GmbH. All rights reserved. (AU)

• FAPESP's process: 11/19067-2 - Study of biofilms associated with reverse osmosis membranes used in wastewater treatment in oil refineries
• Grantee: Virginia Medeiros de Siqueira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral