| Full text | |
| Author(s): |
Monte-Bello, Carolina C.
[1, 2]
;
Araujo, Elias F.
[2, 3]
;
Martins, Marina C. M.
[2]
;
Mafra, Valeria
[4]
;
da Silva, Viviane C. H.
[1, 2]
;
Celente, Viviane
[2]
;
Caldana, Camila
[2, 5]
Total Authors: 7
|
| Affiliation: | [1] Univ Campinas UNICAMP, Campinas, SP - Brazil
[2] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Bioethanol Sci & Technol Lab CTBE, Campinas, SP - Brazil
[3] Univ Vicosa UFV, Vicosa, MG - Brazil
[4] CNPEM, CTBE, Campinas, SP - Brazil
[5] Max Planck Partner Grp, Brazilian Bioethanol Sci & Technol Lab CTBE CNPEM, Vicosa, MG - Brazil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | JOVE-JOURNAL OF VISUALIZED EXPERIMENTS; n. 138 AUG 2018. |
| Web of Science Citations: | 1 |
| Abstract | |
A wide range of studies in plant biology are performed using hydroponic cultures. In this work, an in vitro hydroponic growth system designed for assessing plant responses to chemicals and other substances of interest is presented. This system is highly efficient in obtaining homogeneous and healthy seedlings of the C-3 and C-4 model species Arabidopsis thaliana and Setaria viridis, respectively. The sterile cultivation avoids algae and microorganism contamination, which are known limiting factors for plant normal growth and development in hydroponics. In addition, this system is scalable, enabling the harvest of plant material on a large scale with minor mechanical damage, as well as the harvest of individual parts of a plant if desired. A detailed protocol demonstrating that this system has an easy and low-cost assembly, as it uses pipette racks as the main platform for growing plants, is provided. The feasibility of this system was validated using Arabidopsis seedlings to assess the effect of the drug AZD-8055, a chemical inhibitor of the target of rapamycin (TOR) kinase. TOR inhibition was efficiently detected as early as 30 min after an AZD-8055 treatment in roots and shoots. Furthermore, AZD-8055-treated plants displayed the expected starch-excess phenotype. We proposed this hydroponic system as an ideal method for plant researchers aiming to monitor the action of plant inducers or inhibitors, as well as to assess metabolic fluxes using isotope-labeling compounds which, in general, requires the use of expensive reagents. (AU) | |
| FAPESP's process: | 12/19561-0 - Regulation of plant growth by the Target of rapamycin (TOR) pathway |
| Grantee: | Camila Caldana |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Young Investigators Grants |
| FAPESP's process: | 14/10407-3 - Cross-talk between the Target of Rapamycin (TOR) pathway and energetic metabolism in plants |
| Grantee: | Carolina Cassano Monte Bello |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 14/07918-6 - Functional characterization of the Target of Rapamycin (TOR) gene using Setaria as the model plant: dissecting the role of TOR pathway regulating growth and metabolism in C4 plants |
| Grantee: | Valéria Mafra Cota |
| Support Opportunities: | Scholarships in Brazil - Post-Doctorate |