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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Control of renal sympathetic nerve activity by neurotransmitters in the spinal cord in Goldblatt hypertension

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Milanez, Maycon I. O. [1] ; Nishi, Erika E. [1] ; Sato, Alex Y. S. [1] ; Futuro Neto, Henrique A. [2] ; Bergamaschi, Cassia T. [1] ; Campos, Ruy R. [1]
Total Authors: 6
[1] Univ Fed Sao Paulo, Escola Paulista Med, Cardiovasc Div, Dept Physiol, Rua Botucatu 862, Ground Floor, BR-04023060 Sao Paulo, SP - Brazil
[2] Univ Fed Espirito Santo, Hlth Sci Ctr, Dept Morphol, Vitoria, ES - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Brain Research; v. 1698, p. 43-53, NOV 1 2018.
Web of Science Citations: 1

The role of spinal cord neurons in renal sympathoexcitation remains unclear in renovascular hypertension, represented by the 2-kidney, 1-clip (2K1C) model. Thus, we aimed to assess the influence of spinal glutamatergic and AT1 angiotensin II receptors on renal sympathetic nerve activity (rSNA) in 2K1C Wistar rats. Hypertension was induced by clipping the renal artery with a silver clip. After six weeks, a catheter (PE-10) was inserted into the subarachnoid space and advanced to the T10-11 vertebral level in urethane-anaesthetized rats. The effects of intrathecally (i.t.) injected kynurenic acid (KYN) or losartan (Los) on blood pressure (BP) and rSNA were analysed over 2 consecutive hours. KYN induced a significantly larger drop in rSNA among 2K1C rats than among control (CTL) rats (CTL vs. 2K1C: -8 +/- 3 vs. -52 +/- 9 spikes/s after 120'). Los also evoked a significantly larger drop in rSNA among 2K1C rats than among CTL rats starting at 80' after administration (CTL vs. 2K1C - 80 min: -10 +/- 2 vs. -32 +/- 6{*}; 100 min: -15 +/- 4 vs. -37 +/- 9{*}; 120 min: -12 +/- 5 vs. -37 +/- 8{*} spikes/s). KYN decreased BP similarly in the CTL and 210 C groups; however, Los significantly decreased BP in the 210 C group only. We found upregulation of AT1 gene expression in the T11-12 spinal segments in the 2K1C group but no change in gene expression for AT2 or ionotropic glutamate (NMDA, kainate and AMPA) receptors. Thus, our data show that spinal ionotropic glutamatergic and AT1 receptors contribute to increased rSNA in the 2K1C model, leading to the maintenance of hypertension; however, the participation of spinal AT1 receptors seems to be especially important in the establishment of sympathoexcitation in this model. The origins of those projections, i.e., the brain areas involved in establishing the activity of spinal glutamatergic and angiotensinergic pathways, remain unclear. (C) 2018 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 13/13332-1 - Possible interaction between Angiotensin II and aldosterone in renovascular hypertension in Wistar rats
Grantee:Gisele Silvério Lincevicius
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 13/23741-6 - Evaluation of central mechanisms in the renorenal reflex and the role of afferent renal nerves in renovascular hypertension
Grantee:Erika Emy Nishi
Support type: Scholarships in Brazil - Post-Doctorate