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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Protective Effects of Anti-IL17 on Acute Lung Injury Induced by LPS in Mice

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Righetti, Renato Fraga [1, 2] ; dos Santos, Tabata Maruyama [1, 2] ; Camargo, Leandro do Nascimento [2] ; Rolim Barbosa Aristoteles, Luciana Ritha Cassia [2] ; Fukuzaki, Silvia [2] ; Ribas de Souza, Flavia Castro [2] ; Roncon Santana, Fernanda Paula [2] ; Rodrigues de Agrela, Marcus Vinicius [2] ; Cruz, Maysa Mariana [3] ; Cardoso Alonso-Vale, Maria Isabel [3] ; Genaro, Isabella Santos [2, 4] ; Saraiva-Romanholo, Beatriz Mangueira [5, 2] ; Leick, Edna Aparecida [2] ; Martins, Milton de Arruda [2] ; Prado, Carla Maximo [6] ; Lopes Calvo Tiberio, Iolanda de Fatima [2]
Total Authors: 16
[1] Hosp Sirio Libanes, Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med FMUSP, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Dept Ciencias Biol, Diadema, SP - Brazil
[4] Sao Paulo Hosp IAMSPE, Sao Paulo - Brazil
[5] Univ City Sao Paulo UNICID, Sao Paulo - Brazil
[6] Univ Fed Sao Paulo, Dept Biosci, Santos, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Web of Science Citations: 4

Introduction: T helper 17 (Th17) has been implicated in a variety of inflammatory lung and immune system diseases. However, little is known about the expression and biological role of IL-17 in acute lung injury (ALI).We investigated the mechanisms involved in the effect of anti-IL17 in a model of lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice. Methods: Mice were pre-treated with anti-IL17, 1h before saline/LPS intratracheal administration alongside non-treated controls and levels of exhaled nitric oxide (eNO), cytokine expression, extracellular matrix remodeling and oxidative stress, as well as immune cell counts in bronchoalveolar lavage fluid (BALF), and respiratory mechanics were assessed in lung tissue. Results: LPS instillation led to an increase in multiple cytokines, proteases, nuclear factor-kappa B, and Forkhead box P3 (FOXP3), eNO and regulators of the actomyosin cytoskeleton, the number of CD4+ and iNOS-positive cells as well as the number of neutrophils and macrophages in BALF, resistance and elastance of the respiratory system, ARG-1 gene expression, collagen fibers, and actin and 8-iso-PGF2 alpha volume fractions. Pre-treatment with anti-IL17 led to a significant reduction in the level of all assessed factors. Conclusions: Anti-IL17 can protect the lungs from the inflammatory effects of LPS-induced ALI, primarily mediated by the reduced expression of cytokines and oxidative stress. This suggests that further studies using anti-IL17 in a treatment regime would be highly worthwhile. (AU)

FAPESP's process: 13/17944-1 - Anti-IL-17 in the modulation of lung mechanics, inflammation, oxidative stress, extracellular matrix remodeling in mice with chronic pulmonary inflammation associated or not with acute lung injury induced by LPS acute lung injury by LPS
Grantee:Iolanda de Fátima Lopes Calvo Tibério
Support type: Regular Research Grants