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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Identification of pathogenic and nonpathogenic Leptospira species of Brazilian isolates by Matrix Assisted Laser Desorption/Ionization and Time Flight mass spectrometry

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Author(s):
Karcher, Daniel [1] ; Grenfell, Rafaella C. [1] ; Moreno, Andrea Micke [2] ; Moreno, Luisa Zanolli [2] ; Vasconcellos, Silvio Arruda [2] ; Heinemann, Marcos B. [2] ; de Almeida Junior, Joao N. [3] ; Juliano, Luiz [1] ; Juliano, Maria A. [1]
Total Authors: 9
Affiliation:
[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Biofis, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Fac Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Inst Med Trop Sao Paulo, Lab Micol Med, Div Lab Cent, Hosp Clin, Fac Med, LIM 03, Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Brazilian Journal of Microbiology; v. 49, n. 4, p. 900-908, OCT-DEC 2018.
Web of Science Citations: 0
Abstract

Matrix Assisted Laser Desorption/Ionization and Time of Flight mass spectrometry (MALDITOF MS) is a powerful tool for the identification of bacteria through the detection and analysis of their proteins or fragments derived from ribosomes. Slight sequence variations in conserved ribosomal proteins distinguish microorganisms at the subspecies and strain levels. Characterization of Leptospira spp. by 16S RNA sequencing is costly and time-consuming, and recent studies have shown that closely related species (e.g., Leptospira interrogans and Leptospira kirschneri) may not be discriminated using this technology. Herein, we report an in-house Leptospira reference spectra database using Leptospira reference strains that were validated with a collection of well-identified Brazilian isolates kept in the Bacterial Zoonosis Laboratory at the Veterinary Preventive Medicine and Animal Health Department at Sao Paulo University. In addition, L. interrogans and L. kirschneri were differentiated using an in-depth mass spectrometry analysis with ClinProTools (TM) software. In conclusion, our in-house reference spectra database has the necessary accuracy to differentiate pathogenic and non-pathogenic species and to distinguish L. interrogans and L. kirschneri. (C) 2018 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. (AU)

FAPESP's process: 13/17136-2 - Characterization of Leptospira strains isolated in Brazil by Pulsed Field Gel Electrophoresis (PFGE) typing and comparative genomic analysis
Grantee:Luisa Zanolli Moreno
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/50191-4 - Synthesis, kinetic studies and applications of substrates and inhibitors for proteolytic enzymes.
Grantee:Maria Aparecida Juliano
Support type: Research Projects - Thematic Grants