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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Synthesis and evaluation of a pentafluorobenzamide stationary phase for HPLC separations in the reversed phase and hydrophilic interaction modes

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Author(s):
Ferreira, Claudio de Castro [1] ; Gama, Mariana Roberto [1] ; da Silva, Gisele Silvestre [1] ; Almeida, Wanda Pereira [1] ; Collins, Carol Hollingworth [1] ; Sales Fontes Jardim, Isabel Cristina [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, Inst Chem, Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF SEPARATION SCIENCE; v. 41, n. 20, p. 3855-3862, OCT 2018.
Web of Science Citations: 1
Abstract

A pentafluorobenzamide stationary phase was synthesized by an easy method with no intermediate purification steps. Physicochemical characterization (elemental analysis, fourier transforminfrared spectroscopy, Si-29 and C-13 nuclear magnetic resonance spectroscopy) confirmed the presence of pentafluorobenzamide functionalization on the surface of the silica particles. The pentafluorobenzamide stationary phase proved to be quite versatile as it can be used in two different modes in liquid chromatography: reversed phase and hydrophilic interaction liquid chromatography. Chromatographic characterizations in both modes confirmed the multiple interactions established by the new stationary phase, such as hydrogen bonding and p-p and ion-exchange interactions. The pentafluorobenzamide stationary phase was successfully employed for the separation of nucleosides and antihypertensive drugs under hydrophilic interaction liquid chromatography conditions, as well as pesticides and benzodiazepine using reversed phase conditions. The stationary phase showed significant potential when compared with commercial columns. (AU)

FAPESP's process: 06/57897-9 - Preparation, characterization and application of new stationary phases for high-performance liquid chromatography and electrochromatography
Grantee:Carol Hollingworth Collins
Support type: Research Projects - Thematic Grants