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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

alpha-Linolenic acid prevents hepatic steatosis and improves glucose tolerance in mice fed a high-fat diet

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Goncalves, Natalia Bonissi [1] ; Bannitz, Rafael Ferraz [1] ; Silva, Bruna Ramos [1] ; Becari, Danielle Duran [1] ; Poloni, Carolina [1] ; Gomes, Patricia Moreira [1] ; Foss, Milton Cesar [1] ; Foss-Freitas, Maria Cristina [1]
Total Authors: 8
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Div Endocrinol & Metab, Dept Med, Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Clinics; v. 73, 2018.
Web of Science Citations: 1

OBJECTIVES: Dietary omega-3 fatty acids have been efficacious in decreasing serum cholesterol levels and reducing the risk of cardiovascular disease. However, the metabolic and molecular changes induced by the omega-3 fatty acid oc-linolenic acid (ALA), which is found in linseed oil, are not fully understood. In this study, we showed a correlation between ALA and insulin resistance, inflammation and endoplasmic reticulum stress (ERS). METHODS: We studied 40 male mice (C57/BL6) divided into 4 groups: a control (C) group, a control + omega-3/ALA (CA) group, a high-fat diet (HFD) (H) group and a high-fat diet + omega-3/ALA (HA) group. For 8 weeks, the animals in the H and HA groups were fed a high-fat (60%) diet, while the animals in the C and CA groups received regular chow. The diets of the CA and HA groups were supplemented with 10% lyophilized ALA. RESULTS: ALA supplementation improved glucose tolerance and reduced insulin resistance, as measured by intraperitoneal glucose tolerance tests and the homeostasis model assessment for insulin resistance, respectively. In addition, ALA reduced hepatic steatosis and modified the standard fat concentration in the liver of animals fed an HFD. Dietary ALA supplementation reduced the serum levels of interleukin 6 (IL-6), interleukin 1 beta (IL-1 beta) and monocyte chemoattractant protein-1 (MCP-1), increased the expression of important chaperones such as binding immunoglobulin protein (BIP) and heat shock protein 70 (HSP70) and reduced the expression of C/EBP-homologous protein (CHOP) and X-box binding protein 1 (XBP1) in hepatic tissues, suggesting an ERS adaptation in response to ALA supplementation. CONCLUSIONS: Dietary ALA supplementation is effective in preventing hepatic steatosis; is associated with a reduction in insulin resistance, inflammation and ERS; and represents an alternative for improving liver function and obtaining metabolic benefits. (AU)

FAPESP's process: 11/09640-7 - Metabolic effects of omega-3 fatty acid in insulin resistance conditions
Grantee:Maria Cristina Foss de Freitas
Support type: Research Grants - Young Investigators Grants