Oxytocin effects on osteoblastic differentiation of Bone Marrow Mesenchymal Stem Cells from adult and aging female Wistar rats

Author(s)

Santos, Leandro Figueiredo ^[1] ; Singulani, Monique Patricio ^[1] ; Stringhetta-Garcia, Camila Tami ^[1] ; Penha Oliveira, Sandra Helena ^{[1, 2]} ; Chaves-Neto, Antonio Hernandes ^{[1, 2]} ; Menegati Dornelles, Rita Cassia ^{[1, 2]}

Affiliation

^[1] Unesp SBFis, Programa Posgrad Multictr Ciencias Fisiol, Aracatuba, SP - Brazil

^[2] Sao Paulo State Univ, Sch Dent, Dept Basic Sci, UNESP, Aracatuba - Brazil

• Document type: Journal article
• Source: Experimental Gerontology; v. 113, p. 58-63, NOV 2018.
• Web of Science Citations: 2

Abstract

Recently, it has been suggested that oxytocin (OT) might play a role in the control of bone remodeling and in bone health of young and adult females. The purpose of this study was to evaluate the effect of osteogenic medium (OM) plus OT (OM + OT; 100 nmol/L) on osteoblastic differentiation of bone marrow mesenchymal stem cells (BMMSCs) from cyclic adult (12 months old) and acyclic aging (24 months old) female Wistar rats. After 14 days, OM + OT increased the oxytocin and oxytocin receptor in the BMMSCs from animals of both age groups relative to OM controls. Alkaline phosphatase activity was higher in the OM + OT than OM group in BMMSCs from 24-month-old female rats. OM + OT improved osteogenic differentiation, observed by anticipated mineralization and increased gene expression of bone morphogenetic protein 2, bone sialoprotein, osteopontin and osteocalcin in both aged relative to OM controls. These findings suggest a role for OT as an adjuvant to induce osteoblastic differentiation of BMMSCs from aged female rat. (AU)

• FAPESP's process: 11/15501-0 - Action of oxytocin in the process of osteoblast differentiation in senescent rats
• Grantee: Leandro Figueiredo dos Santos
• Support Opportunities: Scholarships in Brazil - Master