Milk fat protects Bifidobacterium animalis subsp lactis Bb-12 from in vitro gastrointestinal stress in potentially synbiotic table spreads

Author(s)

dos Santos, Clara Simone ^[1] ; Batista de Souza, Cinthia Hoch ^{[1, 2]} ; Padilha, Marina ^{[3, 1]} ; Gioielli, Luiz Antonio ^[1] ; Rodrigues Ract, Juliana Neves ^[1] ; Saad, Susana Marta Isay ^{[3, 1]}

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, Av Prof Lineu Prestes 580, B16, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ North Parana, Dairy Sci & Technol, Rua Marselha 591, BR-86041140 Londrina, PR - Brazil

^[3] Univ Sao Paulo, Food Res Ctr, Sao Paulo - Brazil

• Document type: Journal article
• Source: FOOD & FUNCTION; v. 9, n. 8, p. 4274-4281, AUG 1 2018.
• Web of Science Citations: 3

Abstract

The viability and the in vitro gastrointestinal survival of Bifidobacterium animalis subsp. lactis Bb-12 (Bifidobacterium Bb-12) in table spreads with different proportions of milk fat (MF) and palm olein (PO) (MF : PO 40 : 60 and MF : PO 20 : 80) were investigated for up to 28 days of storage at 5 degrees C. Moreover, qPCR alone and combined with propidium monoazide (PMA) were compared with the traditional plate count method for determining the in vitro gastrointestinal survival of Bifidobacterium Bb-12 in table spreads after 35 days of storage. Formulations showed probiotic viabilities ranging from 8 to 9 log CFU g(-1) across the whole storage period, and the milk fat and palm olein in different concentrations did not affect this viability. Bifidobacterium Bb-12 showed good survival after six hours under in vitro simulated gastrointestinal conditions during the studied storage period, with average reductions of 1.70 (MF : PO 40 : 60) and 2.16 log CFU g(-1) (MF : PO 20 : 80). The results of the qPCR with PMA treatment and the plate count method were similar and the qPCR without PMA treatment was shown to overestimate the Bifidobacterium Bb-12 populations. However, the MF : PO 40 : 60 spread showed a Bb-12 population between 0.76 and 1.43 log CFU g(-1) higher than that of MF : PO 20 : 80. Thus, the results showed that table spreads, especially food matrices with a higher proportion of milk fat, are suitable for the incorporation of Bifidobacterium Bb-12. (AU)

• FAPESP's process: 15/15863-0 - Application of structured lipids for obtaining a spread as a probiotic and prebiotic functional food
• Grantee: Juliana Neves Rodrigues Ract
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/04784-9 - Improvement of probiotic margarine sensory features and evaluation of the product functional potential
• Grantee: Clara Simone dos Santos
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 13/07914-8 - FoRC - Food Research Center
• Grantee: Bernadette Dora Gombossy de Melo Franco
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC