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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A Cell Junctional Protein Network Associated with Connexin-26

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Batissoco, Ana C. [1, 2] ; Salazar-Silva, Rodrigo [2] ; Oiticica, Jeanne [1] ; Bento, Ricardo F. [1] ; Mingroni-Netto, Regina C. [2] ; Haddad, Luciana A. [2]
Total Authors: 6
[1] Univ Sao Paulo, Fac Med, Hosp Clin, Lab Otorrinolaringol LIM32, BR-01246903 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biociencias, Dept Genet & Evolutionary Biol, Human Genome & Stem Cell Res Ctr, BR-05508090 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Web of Science Citations: 2

GJB2 mutations are the leading cause of non-syndromic inherited hearing loss. GJB2 encodes connexin-26 (CX26), which is a connexin (CX) family protein expressed in cochlea, skin, liver, and brain, displaying short cytoplasmic N-termini and C-termini. We searched for CX26 C-terminus binding partners by affinity capture and identified 12 unique proteins associated with cell junctions or cytoskeleton (CGN, DAAM1, FLNB, GAPDH, HOMER2, MAP7, MAPRE2 (EB2), JUP, PTK2B, RAI14, TJP1, and VCL) by using mass spectrometry. We show that, similar to other CX family members, CX26 co-fractionates with TJP1, VCL, and EB2 (EB1 paralogue) as well as the membrane-associated protein ASS1. The adaptor protein CGN (cingulin) co-immuno-precipitates with CX26, ASS1, and TJP1. In addition, CGN co-immunoprecipitation with CX30, CX31, and CX43 indicates that CX association is independent on the CX C-terminus length or sequence. CX26, CGN, FLNB, and DAMM1 were shown to distribute to the organ of Corti and hepatocyte plasma membrane. In the mouse liver, CX26 and TJP1 co-localized at the plasma membrane. In conclusion, CX26 associates with components of other membrane junctions that integrate with the cytoskeleton. (AU)

FAPESP's process: 13/08028-1 - CEGH-CEL - Human Genome and Stem Cell Research Center
Grantee:Mayana Zatz
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 12/50154-1 - Identification of connexin 26 binding proteins
Grantee:Ana Carla Batissoco
Support type: Scholarships in Brazil - Post-Doctorate