Cell-free therapy with the secretome of adipose tissue-derived stem cells in rats' frozen-thawed ovarian grafts

Author(s)

Damous, Luciana Lamarao ^{[1, 2]} ; Teofilo Saturi de Carvalho, Ana Elisa ^[3] ; Nakamuta, Juliana Sanajotti ^[3] ; Shiroma, Marcos Eiji ^[1] ; Sposato Louzada, Andressa Cristina ^[1] ; Soares-, Jr., Jose Maria ^[1] ; Krieger, Jose Eduardo ^[3] ; Baracat, Edmund C. ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Med, Lab Biol Estrutural & Mol LIM58, Disciplina Ginecol, Dr Arnaldo Av 455, 4nf Floor, Room 4119, BR-01246903 Pacaembu, SP - Brazil

^[2] Baturite St 120, Ap 91, BR-01530030 Aclimacao, SP - Brazil

^[3] Univ Sao Paulo, Fac Med, Heart Inst Incor, Lab Genet & Mol Cardiol, Dr Eneas de Carvalho Aguiar Av 44, 10th Floor, BR-05403000 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: STEM CELL RESEARCH & THERAPY; v. 9, NOV 21 2018.
• Web of Science Citations: 0

Abstract

The use of secretome may be a new strand of cell therapy, which is equal to or even superior to the injection of live cells, called cell-free therapy. In ovarian transplantation, this approach may be a therapeutic possibility for the ovarian graft in hypoxia. We designed the present study to evaluate whether the cell-free therapy with the secretome of adipose tissue-derived stem cells (ASCs) in rat frozen-thawed ovarian grafts could protect a graft against ischemic injury. A single dose of rat ASCs secretome or vehicle was injected into the bilateral frozen-thawed ovaries of 18 adult female rats immediately after an autologous transplant. Nine animals were used to control the cryopreservation protocol and were evaluated before and after the cryopreservation process. Daily vaginal smears were performed for estrous cycle evaluation until euthanasia on postoperative day 30. Follicle viability by trypan blue, graft morphology by HE, and apoptosis by TUNEL and cleaved-caspase-3 were assessed. No differences were found with respect to estrous cycle resumption and follicle viability (p > 0.05). However, compared with the vehicle-treated grafts, the morphology of the secretome-treated grafts was impaired, showing reduced follicular population and increased apoptosis (p < 0.05). ASC secretome impaired the rat frozen-thawed ovarian graft from ischemic injury. However, more studies are needed to evaluate the factors involved and the possibility of applying the secretome in scaffolds to optimize its use. (AU)

• FAPESP's process: 12/09469-9 - Cellular and gene therapy in experimental fresh and cryopreserved ovary transplant
• Grantee: Edmund Chada Baracat
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/17897-5 - Cellular and gene therapy in experimental fresh and cryopreserved ovary transplant
• Grantee: Luciana Lamarão Damous
• Support Opportunities: Scholarships in Brazil - Post-Doctoral