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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vitro antibacterial and cytotoxic activities of carvacrol and terpinen-4-ol against biofilm formation on titanium implant surfaces

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Maquera-Huacho, Patricia Milagros [1] ; Tonon, Caroline Coradi [1] ; Correia, Marilia Ferreira [2] ; Francisconi, Renata Serignoli [2] ; Ferreira Bordini, Ester Alves [3] ; Marcantonio, Elcio [1] ; Palomari Spolidorio, Denise Madalena [2]
Total Authors: 7
[1] Sao Paulo State Univ Unesp, Sch Dent, Dept Oral Diag & Surg, Araraquara, SP - Brazil
[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Pathol, Araraquara, SP - Brazil
[3] Sao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: BIOFOULING; v. 34, n. 6, p. 699-709, 2018.
Web of Science Citations: 1

This study evaluated the antibacterial properties of carvacrol and terpinen-4-ol against Porphyromonas gingivalis and Fusobacterium nucleatum and its cytotoxic effects on fibroblast cells. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) were examined. The minimum biofilm inhibition concentration (MBIC) was evaluated by XTT assay. Biofilm decontamination on titanium surfaces was quantified (CFU ml(-1)), evaluated by confocal laser scanning microscopy (CLSM) and cytotoxic activity by MTT. The MIC and MBC for carvacrol were 0.007% and 0.002% for P. gingivalis and F. nucleatum, and 0.06% for terpinen-4-ol for both microorganisms. The MBIC for carvacrol was 0.03% and 0.06% for P. gingivalis and F. nucleatum, and for terpinen-4-ol was 0.06% and 0.24%. The results indicated anti-biofilm activity using carvacrol (0.26%, 0.06%) and terpinen-4-ol (0.95%, 0.24%) and showed cytotoxic activity similar to chlorohexidine (CHX). However, terpinen-4-ol (0.24%) showed higher cell viability than other treatments. Carvacrol and terpinen-4-ol showed antibacterial activity in respect of reducing biofilms. Moreover, CHX-like cytotoxicity was observed. (AU)

FAPESP's process: 09/54035-4 - Facility for advanced studies of biosystems and nanostructured materials
Grantee:Igor Polikarpov
Support type: Multi-user Equipment Program
FAPESP's process: 15/08742-1 - Characterization of Terpinen-4-ol on fungi and bacteria: evaluation of synergic effect/additive, cytotoxicity, adhesion in co-culture and change of virulence factors
Grantee:Denise Madalena Palomari Spolidorio
Support type: Regular Research Grants