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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Relationship of peripheral blood mononuclear cells miRNA expression and parasitic load in canine visceral leishmaniasis

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Bragato, Jaqueline Poleto [1] ; Melo, Larissa Martins [1] ; Venturin, Gabriela Lovizutto [1] ; Rebech, Gabriela Torres [1] ; Garcia, Leandro Encarnacao [2] ; Lopes, Flavia Lombardi [2] ; Felix de Lima, Valeria Marcal [1]
Total Authors: 7
[1] Sao Paulo State Univ, UNESP, Dept Anim Clin Surg & Reprod, Sao Paulo - Brazil
[2] Sao Paulo State Univ, UNESP, Dept Support Prod & Anim Hlth, Sch Vet Med, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: PLoS One; v. 13, n. 12 DEC 5 2018.
Web of Science Citations: 2

Visceral leishmaniasis (VL) in humans is a chronic and often fatal disease if left untreated. Dogs appear to be the main reservoir host for L. infantum infection, however, in many regions other canids such as jackals, foxes, wolves and other mammals, such as hares or black rats, have been implicated as wild reservoirs. Most dogs cannot form an effective immune response against this infection, and this could be modulated by small non-coding RNAs, called microRNAs, responsible for post-transcriptional control of gene expression. Here, we evaluated the expression of miRNAs in peripheral blood mononuclear cells (PBMC) of symptomatic dogs naturally infected with Leishmania (L.) infantum (n = 10) and compared to those of healthy dogs (n = 5). Microarray analysis revealed that miR-21, miR-424, miR-194 and miR-451 had a 3-fold increase in expression, miR-192, miR-503, and miR-371 had a 2-fold increase in expression, whereas a 2-fold reduction in expression was observed for miR-150 and miR-574. Real-time PCR validated the differential expression of miR-21, miR-150, miR-451, miR-192, miR-194, and miR-371. Parasite load of PBMC was measured by real-time PCR and correlated to the differentially expressed miRNAs, showing a strong positive correlation with expression of miR-194, a regular positive correlation with miR-371 expression, and a moderate negative correlation with miR-150 expression in PBMC. These findings suggest that Leishmania infection interferes with miRNAs expression in PBMC, and their correlation with parasite load may help in the identification of therapeutic targets in Canine Visceral Leishmaniasis (CVL). (AU)

FAPESP's process: 15/16972-7 - The role of miRNA in Leishmania infantum infection in mononuclear cells and spleen leukocytes from peripheral blood of dog
Grantee:Valéria Marçal Felix de Lima
Support Opportunities: Regular Research Grants
FAPESP's process: 16/02359-4 - The role of miRNA in the infection of peripheral blood mononuclear cells and splenic leukocytes from dog by Leishmania infantum
Grantee:Jaqueline Poleto Bragato
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 15/16101-6 - The role of miRNAs in mononuclear cells infection of peripheral blood and spleen leukocytes the dog by Leishmania infantum
Grantee:Larissa Martins Melo
Support Opportunities: Scholarships in Brazil - Doctorate