Genetically encodable bioluminescent system from fungi

Author(s):Show less -

Kotlobay, Alexey A. ^[1] ; Sarkisyan, Karen S. ^{[1, 2, 3, 4]} ; Mokrushina, Yuliana A. ^[1] ; Marcet-Houben, Marina ^{[5, 6]} ; Serebrovskaya, Ekaterina O. ^[1] ; Markina, Nadezhda M. ^{[1, 2, 7]} ; Somermeyer, Louisa Gonzalez ^[3] ; Gorokhovatsky, Andrey Y. ^[1] ; Vvedensky, Andrey ^[1] ; Purtov, Konstantin V. ^[8] ; Petushkov, Valentin N. ^[8] ; Rodionova, Natalja S. ^[8] ; Chepurnyh, Tatiana V. ^[1] ; Fakhranurova, Liliia I. ^[9] ; Guglya, Elena B. ^[10] ; Ziganshin, Rustam ^[1] ; Tsarkova, Aleksandra S. ^{[1, 10]} ; Kaskova, Zinaida M. ^{[1, 10]} ; Shender, Victoria ^[1] ; Abakumov, Maxim ^{[10, 11]} ; Abakumova, Tatiana O. ^[12] ; Povolotskaya, Inna S. ^[10] ; Eroshkin, Fedor M. ^[1] ; Zaraisky, Andrey G. ^[1] ; Mishin, Alexander S. ^[1] ; Dolgov, Sergey V. ^[1] ; Mitiouchkina, Tatiana Y. ^{[1, 2]} ; Kopantzev, Eugene P. ^[1] ; Waldenmaier, Hans E. ^[13] ; Oliveira, Anderson G. ^[14] ; Oba, Yuichi ^[15] ; Barsova, Ekaterina ^{[1, 7]} ; Bogdanova, Ekaterina A. ^[1] ; Gabaldon, Toni ^{[5, 6, 16]} ; Stevani, Cassius V. ^[17] ; Lukyanov, Sergey ^{[1, 10]} ; Smirnov, Ivan V. ^[1] ; Gitelson, Josef I. ^[8] ; Kondrashov, Fyodor A. ^[3] ; Yampolsky, Ilia V. ^{[1, 10, 2]}

Affiliation:Show less -

^[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997 - Russia

^[2] Planta LLC, Moscow 121205 - Russia

^[3] IST Austria, A-3400 Klosterneuburg - Austria

^[4] Imperial Coll London, London Inst Med Sci, MRC, London W12 0NN - England

^[5] Barcelona Inst Sci & Technol, Ctr Genom Regulat, Barcelona 08003 - Spain

^[6] Univ Pompeu Fabra, Barcelona 08003 - Spain

^[7] Evrogen JSC, Moscow 117997 - Russia

^[8] Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Fed Res Ctr, Inst Biophys, Krasnoyarsk 660036 - Russia

^[9] Russian Acad Sci, Inst Theoret & Expt Biophys, Moscow 142290 - Russia

^[10] Pirogov Russian Natl Res Med Univ, Moscow 117997 - Russia

^[11] Natl Res Technol Univ MISiS, Biomed Nanomat, Moscow 119049 - Russia

^[12] Skolkovo Inst Sci & Technol, Moscow 121205 - Russia

^[13] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05508000 Sao Paulo - Brazil

^[14] Univ Sao Paulo, Inst Oceanog, Dept Oceanog Fis Quim & Geol, BR-05508120 Sao Paulo - Brazil

^[15] Chubu Univ, Dept Environm Biol, Kasugai, Aichi 4878501 - Japan

^[16] Catalan Inst Res & Adv Studies ICREA, Barcelona 08010 - Spain

^[17] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, BR-05508000 Sao Paulo - Brazil

• Document type: Journal article
• Source: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA; v. 115, n. 50, p. 12728-12732, DEC 11 2018.
• Web of Science Citations: 29

Abstract

Bioluminescence is found across the entire tree of life, conferring a spectacular set of visually oriented functions from attracting mates to scaring off predators. Half a dozen different luciferins, molecules that emit light when enzymatically oxidized, are known. However, just one biochemical pathway for luciferin biosynthesis has been described in full, which is found only in bacteria. Here, we report identification of the fungal luciferase and three other key enzymes that together form the biosynthetic cycle of the fungal luciferin from caffeic acid, a simple and widespread metabolite. Introduction of the identified genes into the genome of the yeast Pichia pastoris along with caffeic acid biosynthesis genes resulted in a strain that is autoluminescent in standard media. We analyzed evolution of the enzymes of the luciferin biosynthesis cycle and found that fungal bioluminescence emerged through a series of events that included two independent gene duplications. The retention of the duplicated enzymes of the luciferin pathway in nonluminescent fungi shows that the gene duplication was followed by functional sequence divergence of enzymes of at least one gene in the biosynthetic pathway and suggests that the evolution of fungal bioluminescence proceeded through several closely related stepping stone nonluminescent biochemical reactions with adaptive roles. The availability of a complete eukaryotic luciferin biosynthesis pathway provides several applications in biomedicine and bioengineering. (AU)

• FAPESP's process: 10/11578-5 - Purification and cloning of a reductase and luciferase from bioluminescent fungi.
• Grantee: Anderson Garbuglio de Oliveira
• Support type: Scholarships in Brazil - Post-Doctorate
• FAPESP's process: 13/16885-1 - Fungal bioluminescence: species survey, mechanistic study & toxicological assays
• Grantee: Cassius Vinicius Stevani
• Support type: Regular Research Grants
• FAPESP's process: 11/10507-0 - Fungal bioluminescence: ecological role, purification and cloning of enzymes
• Grantee: Hans Eugene Waldenmaier
• Support type: Scholarships in Brazil - Doctorate (Direct)