Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms

Author(s)

Rossoni, Rodnei Dennis ^[1] ; Velloso, Marisol dos Santos ^[1] ; de Barros, Patricia Pimentel ^[1] ; de Alvarenga, Janaina Araujo ^[1] ; dos Santos, Jessica Diane ^[1] ; Chipoletti dos Santos Prado, Ana Carolina ^[1] ; Ribeiro, Felipe de Camargo ^[1] ; Anbinder, Ana Lia ^[1] ; Junqueira, Juliana Campos ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Inst Sci & Technol, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP - Brazil

• Document type: Journal article
• Source: Microbial Pathogenesis; v. 123, p. 361-367, OCT 2018.
• Web of Science Citations: 3

Abstract

Probiotics can release bioactive substances that can inhibit the growth and biofilm formation of pathogenic microorganisms such as Streptococcus mutans. In this context, we evaluated whether the supernatants of Lactobacillus strains isolated from caries-free subjects can inhibit S. mutans, one of the most important bacteria for dental caries. First, the supernatants of 22 Lactobacillus strains were screened for antibacterial activity against S. mutans in planktonic cultures. All 22 Lactobacillus strains studied (100%) showed antibacterial activity. Thereafter, the Lactobacillus strains with the greatest reductions in the planktonic S. mutans cultures were tested on biofilms. The L. fermentum 20.4, L. paracasei 11.6, L. paracasei 20.3 and L. paracasei 25.4 strains could significantly reduce the number of S. mutans cells in biofilms formed in hydroxyapatite (p < 0.05). This reduction was also confirmed by scanning electron microscopy analysis and was not caused by the decreased pH value in the medium (p > 0.05). In addition, the supernatants of these probiotic strains could also reduce the total biomass of S. mutans biofilms (p < 0.05). In conclusion, most of the Lactobacillus strains tested have some antibacterial activity against S. mutans. L. fermentum 20.4, L. paracasei 11.6, L. paracasei 20.3 and L. paracasei 25.4 produce bioactive substances that caused a significant reduction in S. mutans biofilms. (AU)

• FAPESP's process: 14/11841-9 - Effects of Lactobacillus spp. isolated from oral cavity of caries-free subjects on Streptococcus mutans: study of probiotic activity
• Grantee: Marisol dos Santos Velloso
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 13/25181-8 - Isolation and characterization of Lactobacillus spp. from oral cavity and their probiotic action in Candida albicans: biofilm formation, infection in invertebrate models and EFG1, HWP1 and ALS1 gene expression
• Grantee: Rodnei Dennis Rossoni
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/19219-3 - Identification of bioactive substances produced by Lactobacillus paracasei 28.4 for the control of oral candidiasis
• Grantee: Rodnei Dennis Rossoni
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/09770-9 - Isolation and characterization of Lactobacillus spp. from oral cavity and its probiotic action in Candida albicans and Streptococcus mutans
• Grantee: Juliana Campos Junqueira
• Support Opportunities: Regular Research Grants