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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Complete urological evaluation including sperm DNA fragmentation in male systemic lupus erythematosus patients

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Author(s):
Tiseo, B. C. [1, 2] ; Bonfa, E. [3] ; Borba, E. F. [3] ; Munhoz, G. A. [3] ; Wood, G. J. A. [2] ; Srougi, M. [2] ; Silva, C. A. [3, 4] ; Cocuzza, M. [2]
Total Authors: 8
Affiliation:
[1] Hosp Israelita Albert Einstein, Sao Paulo - Brazil
[2] Hosp Clin HCFMUSP, Discipline Urol, Sao Paulo - Brazil
[3] Hosp Clin HCFMUSP, Div Rheumatol, Sao Paulo - Brazil
[4] Hosp Clin HCFMUSP, Pediat Rheumatol Unit, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Lupus; v. 28, n. 1, p. 59-65, JAN 2019.
Web of Science Citations: 1
Abstract

Objective To evaluate sperm DNA fragmentation analysis in non-azoospermic male systemic lupus erythematosus (SLE) patients. Methods Twenty-eight consecutive male SLE patients (American College of Rheumatology criteria) and 34 healthy controls were evaluated for demographic/exposures data, urological evaluation, hormone profile and sperm analysis (including sperm DNA fragmentation). Clinical features, disease activity/damage scores and treatment were also evaluated. Results The median age (33 (20-52) vs. 36.5 (25-54) years, P = 0.329) and frequency of varicocele (25% vs. 32%, P = 0.183) were similar in SLE patients and healthy controls. Sperm DNA fragmentation showed significantly higher levels of cells class III (44 (9-88) vs. 16.5 (0-80)%, P = 0.001) and cell class IV (10.5 (3-86) vs. 7 (0-36)%, P = 0.039) in SLE. The sperm DNA fragmentation index was also significantly higher in SLE patients (62 (31-97) vs. 25.5 (0-100)%, P < 0.001). Conventional sperm parameters (including sperm count, motility and morphology) were similar in both groups. In SLE patients no correlations were observed between sperm DNA fragmentation index and age, disease duration, Systemic Lupus Erythematosus Disease Activity Index 2000 and Systemic Lupus International Collaborating Clinics/American College of Rheumatology Damage Index scores, and cumulative dose of prednisone, hydroxychloroquine, intravenous cyclophosphamide, methotrexate, azathioprine and mycophenolate mofetil (P > 0.05). Further analysis of SLE patients treated with and without intravenous cyclophosphamide showed that total sperm motility was significantly lower in the former group (64% (15-83) vs. 72% (57-86), P = 0.024). The sperm DNA fragmentation index was alike in both groups (52.5 (31-95) vs. 67.5 (34-97)%, P = 0.185). Conclusions To our knowledge, this is the first demonstration that male non-azoospermic SLE patients have increased sperm DNA fragmentation without evident gonadal dysfunction. Intravenous cyclophosphamide does not seem to be a major determinant for this abnormality. Future prospective study is necessary to determine the impact of this alteration in these patients' fertility. (AU)

FAPESP's process: 15/03756-4 - Assessment of relevance of blood levels of drugs in the monitoring rheumatic autoimmune diseases: safety, effectiveness and adherence to therapy
Grantee:Eloisa Silva Dutra de Oliveira Bonfá
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/14806-0 - Evaluation of ovarian reserve, antibody anti-corpus luteum, cervicovaginal cytology, lower genital tract infections, metabolomics and ovulatory dysfunction in women with juvenile idiopathic arthritis
Grantee:Clovis Artur Almeida da Silva
Support Opportunities: Regular Research Grants