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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The initial stages of oogenesis and their relation to differential fertility in the honey bee (Apis mellifera) castes

Full text
Author(s):
Tanaka, E. D. [1] ; Hartfelder, K.
Total Authors: 2
Affiliation:
[1] Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto. Departamento de Biologia - Brasil
Total Affiliations: 2
Document type: Journal article
Source: ARTHROPOD STRUCTURE & DEVELOPMENT; v. 33, n. 4, p. 431-442, Oct. 2004.
Field of knowledge: Biological Sciences - Morphology
Abstract

Neither the overall differences in ovariole number nor the caste-specifically modulated expression of vitellogenin can fully explain the striking caste differences in honey bee reproduction, in particular the mechanisms that block oogenesis in virgin queens and in workers kept in the presence of a queen. For this reason we investigated the initial stages of oogenesis in queens in relation to mating status and in workers exposed to different social conditions. A striking feature in ovarioles of both castes was a considerably elongated terminal filament which consisted not only of normal terminal filament cells but also contained apparently undifferentiated cells that were tentatively considered as stem cells. BrdU incorporation was detected in the upper germarium, as well as in the terminal filament. Cytoskeleton analysis by TRITC-phalloidin labeling for F-actin, and immunofluorescence detection for â-tubulin did not reveal structural differences in the early oogenesis steps between queens and queenless workers. In contrast, queenright workers showed signs of a disorganized microtubule and microfilament system that could explain the histological evidence for progressive cell death observed in the germaria. In addition to cytoplasmic tubulin we also detected marked intranuclear foci indicating the presence of nuclear âII-tubulin. (AU)

FAPESP's process: 99/00719-6 - Africanized honeybees: an integrated analysis of the africanization process of Apis mellifera, focusing on determinants in fertility of drones, queens and workers
Grantee:Zilá Luz Paulino Simões
Support type: Research Projects - Thematic Grants