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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Comparison between treatment naive juvenile and adult dermatomyositis muscle biopsies: difference of inflammatory cells phenotyping

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Shinjo, Samuel Katsuyuki [1] ; Elias Sallum, Adriana Maluf [2] ; Oba-Shinjo, Sueli Mieko [3] ; Silva, Marilda Guimaraes [1] ; Silva, Clovis Artur [2] ; Nagahashi Marie, Suely Kazue [3]
Total Authors: 6
[1] Univ Sao Paulo, Disciplina Reumatol, Fac Med, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Crianca, Fac Med, Hosp Clin, Sao Paulo - Brazil
[3] Univ Sao Paulo, Lab Biol Mol & Celular, Fac Med, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ADVANCES IN RHEUMATOLOGY; v. 58, OCT 26 2018.
Web of Science Citations: 0

Background: Different inflammatory cells (i.e., CD4, CD8, CD20 and CD68) are involved in pathogenesis of DM muscle. In this context, the aim of this study was to assess and compare these inflammatory cell phenotyping in muscle samples of treatment naive juvenile and adult patients with dermatomyositis. Methods: This is a cross-sectional study, in which 28 untreated juvenile and 28 adult untreated dermatomyositis patients were included. Immunohistochemical analysis was performed on serial frozen muscle sections. Inflammatory cell phenotyping was analyzed quantitatively in endomysium, perimysium, and perivascular (endomysium and perimysium) area. Results: Mean age at disease onset was 73 and 42.0 years in juvenile and adult dermatomyositis, respectively. Both groups had comparable time duration from symptom's onset to biopsy performance. CD4 and CD8 positive cells distributions were similar in both groups in all analyzed area, except for more predominance of CD4 in perimysium at juvenile muscle biopsies. The CD20 and CD68 positive cells were predominantly observed in adult muscle biopsy sections, when compared to juvenile samples, except for similar distribution of CD20 in perivascular endomysium, and CD68 in perimysium. Conclusions: These data show that the differences between juvenile and adult dermatomyositis may be restricted not only to patients' age, but also to different inflammatory cell distribution, particularly, in new-onset disease. Further studies are necessary to confirm the present study data and to analyze meaning of the different inflammatory cell phenotyping distribution finding in these both diseases. (AU)

FAPESP's process: 14/09079-1 - Impact of corticosteroids in the inflammatory process of muscle biopsies of dermatomyositis and polymyositis
Grantee:Samuel Katsuyuki Shinjo
Support type: Regular Research Grants