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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Altered Proteins in the Hippocampus of Patients with Mesial Temporal Lobe Epilepsy

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Author(s):
Persike, Daniele Suzete [1, 2] ; Marques-Carneiro, Jose Eduardo [1, 3] ; de Lima Stein, Mariana Leao [4] ; Targas Yacubian, Elza Marcia [1] ; Centeno, Ricardo [1] ; Canzian, Mauro [5] ; da Silva Fernandes, Maria Jose [1]
Total Authors: 7
Affiliation:
[1] Univ Fed Sao Paulo UNIFESP, Dept Neurol Neurocirurgia, Escola Paulista Med, Rua Pedro de Toledo 669, BR-04039032 Sao Paulo - Brazil
[2] Univ Dohuk UoD, Coll Pharm, Dept Med Chem, 1006AJ, Duhok Duhok, Kurdistan Regio - Iraq
[3] INSERM, Neuropsychol Cognit & Physiopathol Schizophrenia, U1114, 1 Pl Hop, F-67091 Strasbourg - France
[4] Univ Fed Sao Paulo, Dept Microimunoparasito, Disciplina Biol Celular, Escola Paulista Med, BR-04039032 Sao Paulo - Brazil
[5] FMUSP, Inst Coracao INCOR, Dept Anat Patol, BR-04039032 Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: PHARMACEUTICALS; v. 11, n. 4 DEC 2018.
Web of Science Citations: 3
Abstract

Mesial temporal lobe epilepsy (MTLE) is usually associated with drug-resistant seizures and cognitive deficits. Efforts have been made to improve the understanding of the pathophysiology of MTLE for new therapies. In this study, we used proteomics to determine the differential expression of proteins in the hippocampus of patients with MTLE compared to control samples. By using the two-dimensional electrophoresis method (2-DE), the proteins were separated into spots and analyzed by LC-MS/MS. Spots that had different densitometric values for patients and controls were selected for the study. The following proteins were found to be up-regulated in patients: isoform 1 of serum albumin (ALB), proton ATPase catalytic subunit A (ATP6V1A), heat shock protein 70 (HSP70), dihydropyrimidinase-related protein 2 (DPYSL2), isoform 1 of myelin basic protein (MBP), and dihydrolipoamide S-acethyltransferase (DLAT). The protein isoform 3 of the spectrin alpha chain (SPTAN1) was down-regulated while glutathione S-transferase P (GSTP1) and protein DJ-1 (PARK7) were found only in the hippocampus of patients with MTLE. Interactome analysis of the nine proteins of interest revealed interactions with 20 other proteins, most of them involved with metabolic processes (37%), presenting catalytic activity (37%) and working as hydrolyses (25%), among others. Our results provide evidence supporting a direct link between synaptic plasticity, metabolic disturbance, oxidative stress with mitochondrial damage, the disruption of the blood-brain barrier and changes in CNS structural proteins with cell death and epileptogenesis in MTLE. Besides this, the presence of markers of cell survival indicated a compensatory mechanism. The over-expression of GSTP1 in MTLE could be related to drug-resistance. (AU)

FAPESP's process: 08/00068-6 - Proteomic evaluation of temporal lobe epilepsy in human and rat sujected to pilocarpine model, receiving or not neuroprotector agent R-PIA pretreatment.
Grantee:Daniele Suzete Persike
Support Opportunities: Scholarships in Brazil - Post-Doctoral