| Full text | |
| Author(s): |
Cieslarova, Zuzana
[1]
;
Magaldi, Mariana
[2]
;
Barros, Lucelia Alcantara
[3]
;
do Lago, Claudimir Lucio
[1]
;
Oliveira, Daniel Rossado
[1]
;
Helfenstein Fonseca, Francisco Antonio
[4]
;
Izar, Maria Cristina
[4]
;
Lopes, Aline Soriano
[3]
;
Maggi Tavares, Marina Franco
[1]
;
Klassen, Aline
[3]
Total Authors: 10
|
| Affiliation: | [1] Univ Sao Paulo, Inst Chem, Sao Paulo, SP - Brazil
[2] Fed Univ Sao Paulo UNIFESP, Dept Pharmaceut Sci, Diadema, SP - Brazil
[3] Fed Univ Sao Paulo UNIFESP, Dept Chem, Rua Prof Arthur Riedel 275, BR-09972270 Diadema, SP - Brazil
[4] Fed Univ Sao Paulo UNIFESP, Dept Med, Div Cardiol, Sao Paulo, SP - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Journal of Chromatography A; v. 1583, p. 136-142, JAN 4 2019. |
| Web of Science Citations: | 4 |
| Abstract | |
A capillary electrophoresis with diode array and tandem mass spectrometry detection (CE-UV-MS/MS) method has been developed for the targeted assessment of cardiovascular biomarkers candidates, trimethylamine-N-Oxide (TMAO) and L-carnitine, and creatinine in human urine samples. The dual detection was applied due to the high concentration of creatinine (monitored by UV detection at 200 nm) in relation to TMAO and t-carnitine (quantified by selected reaction monitoring (SRM) mass spectrometry), in human urine. All instrumental parameters, sheath liquid (SHL) and background electrolyte (BGE) compositions were optimized with a pool of urine provided by adult healthy volunteers and evaluated by signal-to-noise ratio (SNR) and peak shape of TMAO. The compositions for the optimized BGE was formic acid at concentration of 0.10 mol L-1, and for SHL was 70:30 MeOH:H2O containing 0.05% (v/v) formic acid, delivered at a flow rate of 5 mu L min(-1). Limits of detection for TMAO, L-carnitine and creatinine were 0.76, 0.54 and 303 mu mol L-1, respectively. Limits of quantification were 2.5, 1.8 and 1000 mu mol L-1, respectively. Linearity was evaluated by ANOVA and presented R-2 from 0.993 to 0.997. Precision and accuracy were evaluated at three concentration levels. Coefficients of variation (CV) from 1 to 21% were obtained for the intra-day precision evaluation and from 2 to 16% for the inter-day precision evaluation. The recovery ranged from 75 to 116%. Quantitation of TMAO and L-carnitine in infarcted patients urine in comparison to healthy individuals indicated a 2.2 fold increase of TMAO and a 7.0 fold increase of L-carnitine. These results showed the potential applicability of the proposed method for the evaluation of TMAO and L-carnitine in urine within a panel of candidate metabolites in targeted metabolomics studies of cardiovascular diseases among other conditions. (C) 2018 Elsevier B.V. All rights reserved. (AU) | |
| FAPESP's process: | 12/51692-7 - Role of adaptative immunity on the progress of ischemic heart disease after acute myocardial infarction |
| Grantee: | Francisco Antonio Helfenstein Fonseca |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 12/06642-1 - Formation and properties of adducts of organic hydroxy-compounds with carbonate in aqueous medium and biological conditions |
| Grantee: | Claudimir Lucio Do Lago |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 17/27059-6 - Expanding the metabolic coverage using ion chromatography-mass spectrometry in metabolomics studies of immunosuppressive therapies in renal transplantation |
| Grantee: | Marina Franco Maggi Tavares |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 16/19045-2 - Determination of adenosine and differential protein after acute myocardial infartion |
| Grantee: | Lucelia Alcantara Barros |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |