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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Insights into the Effects of Crack Abuse on the Human Metabolome Using a NMR Approach

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Author(s):
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Costa, Tassia B. B. C. [1] ; Lacerda, Acioly L. T. [2, 3] ; Dal Mas, Caroline [2] ; Brietzke, Elisa [2] ; Pontes, Joao G. M. [1] ; Marins, Lucas A. N. [2] ; Martins, Lucas G. [1] ; Nunes, Marcel V. [3] ; Pedrini, Mariana [2] ; Carvalho, Michelle S. C. [2] ; Mitrovitch, Milan P. [3] ; Hayashi, Mirian A. F. [2] ; Saldanha, Natalia L. [3] ; Poppi, Ronei J. [1] ; Tasic, Ljubica [1]
Total Authors: 15
Affiliation:
[1] Univ Estadual Campinas UNICAMP, Inst Chem, Campinas, SP - Brazil
[2] Univ Fed Sao Paulo UNIFESP, Sao Paulo - Brazil
[3] Inst Bairral Psiquiatria, Ctr Res & Clin Trials Sinapse Bairral, Itapira - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF PROTEOME RESEARCH; v. 18, n. 1, p. 341-348, JAN 2019.
Web of Science Citations: 1
Abstract

Approximately 255 million people consume illicit drugs every year, among which 18 million use cocaine. A portion of this drug is represented by crack, but it is difficult to estimate the number of users since most are marginalized. However, there are no recognized efficacious pharmacotherapies for crack-cocaine dependence. Inflammation and infection in cocaine users may be due to behavior adopted in conjunction with drug-related changes in the brain. To understand the metabolic changes associated with the drug abuse disorder and identify biomarkers, we performed a H-1 NMR-based metabonomic analysis of 44 crack users' and 44 healthy volunteers' blood serum. The LDA model achieved 98% of accuracy. From the water suppressed H-1 NMR spectra analyses, it was observed that the relative concentration of lactate was higher in the crack group, while long chain fatty acid acylated carnitines were decreased, which was associated with their nutritional behavior. Analyses of the aromatic region of CPMG H-1 NMR spectra demonstrated histidine and tyrosine levels increased in the blood serum of crack users. The reduction of carnitine and acylcarnitines and the accumulation of histidine in the serum of the crack users suggest that histamine biosynthesis is compromised. The tyrosine level points to altered dopamine concentration. (AU)

FAPESP's process: 14/18938-8 - Metabolomic profiling of severe mental disorders using Nuclear Magnetic Ressonance (NMR)
Grantee:Ljubica Tasic
Support type: Regular Research Grants
FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
Grantee:Lauro Tatsuo Kubota
Support type: Research Projects - Thematic Grants