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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genotoxic and cytotoxic effects of neolignans isolated from Nectandra leucantha (Lauraceae)

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de Sousa, Fernanda S. [1] ; Nunes, Emilene A. [2] ; Gomes, Kaio S. [2] ; Cerchiaro, Giselle [2] ; Lago, Joao Henrique G. [2]
Total Authors: 5
[1] Univ Fed Sao Paulo, Inst Environm, Chem & Pharmaceut Sci, Diadema, SP - Brazil
[2] Fed Univ ABC, Ctr Nat & Human Sci, Santo Andre, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: TOXICOLOGY IN VITRO; v. 55, p. 116-123, MAR 2019.
Web of Science Citations: 0

In this work was evaluated the cytotoxic activity of dehydrodieugenol B (1) and methyldehydrodieugenol B (2) isolated from Nectandra leucantha (Lauraceae) through cytokinesis-block micronucleus (CBMN) and Comet assay. Compounds 1 and 2 displayed in vitro toxicity against human melanoma cells (SKMEL-147) with IC50 values of 4.4 and 43.6 mu g/mL, respectively. The interaction of these compounds with resistant human melanoma cell line SKMEL-29 was also investigated. Obtained results showed a concentration-response relationship for DNA damage (DI and D%) in SKMEL-29 cells for compounds 1 and 2 causing an increase in DNA damage on their lower concentrations. Concerning the cytokinesis-block micronucleus cytome (CBMNCyt) assay, all treatments demonstrated an increase in cytostatic and cytotoxic indexes. In micronucleus quantification, compound 1 displayed higher index in comparison to control. Regarding necrotic and apoptotic cells, treatments with SKMEL-29 cells demonstrated 100% of cell death induced by compounds 1 and 2 at 25 and 88 mu g/mL, respectively. Additionally, it was observed that apoptosis is prevalent in SKMEL-147 cells treated with compound 1, while necrotic cells were observed in SKMEL-29 cells treated with both compounds. In conclusion, compounds 1 and 2 are suggested as promising cytotoxic agents against human melanoma resistant cells, emphasizing the potential use of these neolignans for the treatment of melanoma. (AU)

FAPESP's process: 15/11936-2 - Use of chemodiversity of plant species in remaining areas of Atlantic Forest from São Paulo State in the selection of biologically active prototypes
Grantee:João Henrique Ghilardi Lago
Support type: Regular Research Grants
FAPESP's process: 14/24475-0 - Studies on the action of SOD1 and metal ions in the damage to biomolecules in neuronal cell culture under oxidative stress and neurodegeneration condition
Grantee:Emilene Arusievicz Nunes
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 15/04143-6 - Naturals and semisynthetics metabolites from twigs of Nectandra leucantha Ness & Mart. (Lauraceae): molecular characterization, potential evaluation and mechanism antitumoral against melanoma
Grantee:Fernanda Samara de Sousa
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 18/07885-1 - Biomolecules from plant species of remnant areas of the Atlantic Forest and Cerrado to treat neglected tropical diseases - chemical and pharmacological aspects
Grantee:João Henrique Ghilardi Lago
Support type: BIOTA-FAPESP Program - Regular Research Grants