| Full text | |
| Author(s): |
de Sousa, Fernanda S.
[1]
;
Nunes, Emilene A.
[2]
;
Gomes, Kaio S.
[2]
;
Cerchiaro, Giselle
[2]
;
Lago, Joao Henrique G.
[2]
Total Authors: 5
|
| Affiliation: | [1] Univ Fed Sao Paulo, Inst Environm, Chem & Pharmaceut Sci, Diadema, SP - Brazil
[2] Fed Univ ABC, Ctr Nat & Human Sci, Santo Andre, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | TOXICOLOGY IN VITRO; v. 55, p. 116-123, MAR 2019. |
| Web of Science Citations: | 0 |
| Abstract | |
In this work was evaluated the cytotoxic activity of dehydrodieugenol B (1) and methyldehydrodieugenol B (2) isolated from Nectandra leucantha (Lauraceae) through cytokinesis-block micronucleus (CBMN) and Comet assay. Compounds 1 and 2 displayed in vitro toxicity against human melanoma cells (SKMEL-147) with IC50 values of 4.4 and 43.6 mu g/mL, respectively. The interaction of these compounds with resistant human melanoma cell line SKMEL-29 was also investigated. Obtained results showed a concentration-response relationship for DNA damage (DI and D%) in SKMEL-29 cells for compounds 1 and 2 causing an increase in DNA damage on their lower concentrations. Concerning the cytokinesis-block micronucleus cytome (CBMNCyt) assay, all treatments demonstrated an increase in cytostatic and cytotoxic indexes. In micronucleus quantification, compound 1 displayed higher index in comparison to control. Regarding necrotic and apoptotic cells, treatments with SKMEL-29 cells demonstrated 100% of cell death induced by compounds 1 and 2 at 25 and 88 mu g/mL, respectively. Additionally, it was observed that apoptosis is prevalent in SKMEL-147 cells treated with compound 1, while necrotic cells were observed in SKMEL-29 cells treated with both compounds. In conclusion, compounds 1 and 2 are suggested as promising cytotoxic agents against human melanoma resistant cells, emphasizing the potential use of these neolignans for the treatment of melanoma. (AU) | |
| FAPESP's process: | 15/11936-2 - Use of chemodiversity of plant species in remaining areas of Atlantic Forest from São Paulo State in the selection of biologically active prototypes |
| Grantee: | João Henrique Ghilardi Lago |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/24475-0 - Studies on the action of SOD1 and metal ions in the damage to biomolecules in neuronal cell culture under oxidative stress and neurodegeneration condition |
| Grantee: | Emilene Arusievicz Nunes |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 15/04143-6 - Naturals and semisynthetics metabolites from twigs of Nectandra leucantha Ness & Mart. (Lauraceae): molecular characterization, potential evaluation and mechanism antitumoral against melanoma |
| Grantee: | Fernanda Samara de Sousa |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 18/07885-1 - Biomolecules from plant species of remnant areas of the Atlantic Forest and Cerrado to treat neglected tropical diseases - chemical and pharmacological aspects |
| Grantee: | João Henrique Ghilardi Lago |
| Support Opportunities: | BIOTA-FAPESP Program - Regular Research Grants |