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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The expression of gene ANKRD1 correlates with hypoxia status in muscle biopsies of treatment-näive adult dermatomyositis

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Author(s):
Samuel Katsuyuki Shinjo ; Sueli Mieko Oba-Shinjo ; Miyuki Uno ; Suely Kazue Nagahashi Marie
Total Authors: 4
Document type: Journal article
Source: MedicalExpress (São Paulo, online); v. 4, n. 4, p. -, Ago. 2017.
Abstract

OBJECTIVES. The ANKRD1 gene codes for the ankyrin repeat domain containing protein 1 and has an important role in myogenesis and possibly also in angiogenesis. Microvasculopathy is a cornerstone and an early pathological marker of change in dermatomyositis, leading to hypoxia and muscle perifascicular atrophy. These alterations could upregulate genes involved in myogenesis and angiogenesis such as ANKRD1. Therefore, we analyzed ANKRD1 expression in muscle biopsies of dermatomyositis and correlated with other hypoxia parameters and with histological changes. METHODS. Total RNA was extracted from frozen muscle biopsies samples of 29 dermatomyositis patients. A control group consisted of 20 muscle biopsies from adult patients with non-inflammatory myopathy diseases. The gene coding for hypoxia-inducible factor 1, alpha subunit (HIF1A), was analyzed to estimate the degree of hypoxia. ANKRD1 and HIF1A transcript expression levels were determined by quantitative real time PCR. RESULTS. Significantly higher ANKRD1 and HIF1A expression levels were observed in dermatomyositis relative to the control group (P<0.001, both genes). In addition, ANKRD1 and HIF1A were coexpressed (r=0.703, P=0.001) and their expression levels correlated positively to perifascicular atrophy (r=0.420, P=0.023 and r=0.404, P=0.030, respectively). CONCLUSIONS. Our results demonstrate ANKRD1 overexpression in dermatomyositis correlated to HIF1A expression and perifascicular atrophy. ANKRD1 involvement in myogenesis and angiogenesis mechanisms indicates that further investigation is worthwhile. (AU)

FAPESP's process: 14/09079-1 - Impact of corticosteroids in the inflammatory process of muscle biopsies of dermatomyositis and polymyositis
Grantee:Samuel Katsuyuki Shinjo
Support type: Regular Research Grants
FAPESP's process: 12/09633-3 - Gene expression of proinflammatory cytokines in muscle biopsies of patients with dermatomyositis/polymyositis: correlation with clinical and laboratory features and outcome
Grantee:Samuel Katsuyuki Shinjo
Support type: Regular Research Grants