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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Serodiagnosis of neurocysticercosis in patients with epileptic seizure using ELISA and immunoblot assay

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Author(s):
Maria M.I. Ishida ; Regina Helena S. Peralta ; José. A. Livramento ; Sumie Hoshino-Shimizu ; José M. Peralta ; Adelaide J. Vaz
Total Authors: 6
Document type: Journal article
Source: Revista do Instituto de Medicina Tropical de São Paulo; v. 48, n. 6, p. -, Dez. 2006.
Abstract

Sera from 88 patients from Santa Catarina and São Paulo states of Brazil, with epileptic seizures who underwent cerebral computed tomography (CT) were analyzed for the detection of antibodies to T. solium cysticercus by ELISA and Immunoblot (IB) with the following antigens: Taenia solium cysticercus total saline (Tso), Taenia crassiceps cysticercus vesicular fluid (Tcra-vf) and T. crassiceps cysticercus glycoproteins (Tcra-gp). ELISA carried out with Tso, Tcra-vf and Tcra-gp antigens showed 95%, 90% and 80% sensitivities, respectively, and 68%, 85% and 93% specificities, respectively. In the epileptic patients group, ELISA positivity was 30%, 51% and 35% with Tso, Tcra-vf and Tcra-gp antigens respectively. Considering the IB as the confirmatory test, the positivity was 16% (14/88) in the epileptic patients total group and 22% (12/54) in the epileptic patients with positive CT and signals of cysticercosis. We found a significant statistical correlation among ELISA or IB results and the phase of the disease when any antigens were used (p < 0.05). We emphasize the need to introduce in the laboratory routine the search for neurocysticercosis (NC) in patients presenting with epileptic seizures because of the high risk of acquiring NC in our region and its potential cause of epilepsy. (AU)

FAPESP's process: 02/12061-0 - Cysticercosis: immunidiagnosis, antigens, immune response, clinical and epidemiologic aspects
Grantee:Adelaide José Vaz
Support type: Research Projects - Thematic Grants