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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A new disposable microfluidic electrochemical paper-based device for the simultaneous determination of clinical biomarkers

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Author(s):
Cincotto, Fernando H. [1, 2] ; Fava, Elson L. [2] ; Moraes, Fernando C. [2] ; Fatibello-Filho, Orlando [2] ; Faria, Ronaldo C. [2]
Total Authors: 5
Affiliation:
[1] Univ Fed Rio de Janeiro, Inst Chem, Rio De Janeiro, RJ - Brazil
[2] Univ Fed Sao Carlos, Chem Dept, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Talanta; v. 195, p. 62-68, APR 1 2019.
Web of Science Citations: 7
Abstract

A new disposable microfluidic electrochemical paper-based device (ePAD) consisting of two spot sensors in the same working electrode for the simultaneous determination of uric acid and creatinine was developed. The spot 1 surface was modified with graphene quantum dots for direct uric acid oxidation and spot 2 surface modified with graphene quantum dots, creatininase and a ruthenium electrochemical mediator for creatinine oxidation. The ePAD was employed to construct an electrochemical sensor (based on square wave voltammetry analysis) for the simultaneous determination of uric acid and creatinine in the 0.010-3.0 mu mol L-1 range. The device showed excellent analytical performance with a very low simultaneous detection limit of 8.4 nmol L-1 to uric acid and 3.7 nmol L-1 to creatinine and high selectivity. The ePAD was applied to the rapid and successful determination of those clinical biomarkers in human urine samples. (AU)

FAPESP's process: 16/12926-3 - Removal and real time determination of environmental pollutants using electrochemical photoassisted system
Grantee:Fernando Cruz de Moraes
Support type: Regular Research Grants
FAPESP's process: 16/16565-5 - Development of ultra-sensitive methods for RNA detection for the diagnosis of Head and Neck Cancer
Grantee:Fernando Henrique Cincotto
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/10012-4 - Development of a disposable biosensors array with a microfluidic device for multiplex determination of species of biological interest in different matrices
Grantee:Elson Luiz Fava
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 17/10118-0 - Study and application of electrochemical technology for the analysis and degradation of endocrine interferents: materials, sensors, processes and scientific dissemination
Grantee:Marcos Roberto de Vasconcelos Lanza
Support type: Research Projects - Thematic Grants