Proteomics of acquired pellicle in gastroesophageal reflux disease patients with or without erosive tooth wear

Author(s):Show less -

Martini, Tatiana ^[1] ; Rios, Daniela ^[1] ; Silva Cassiano, Luiza Paula ^[1] ; de Souza Silva, Cintia Maria ^[1] ; Taira, Even Akemi ^[1] ; Silva Ventura, Talita Mendes ^[1] ; Barbosa Silva Pereira, Heloisa Aparecida ^[1] ; Magalhaes, Ana Carolina ^[1] ; Carvalho, Thiago Saads ^[2] ; Baumann, Tommy ^[2] ; Lussi, Adrian ^[2] ; Oliveira, Ricardo Brandt ^[3] ; Palma-Dibb, Regina Guenka ^[4] ; Rabelo Buzalaf, Marilia Afonso ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Al Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Univ Bern, Dept Restorat Prevent & Pediat Dent, Freiburgstr 7, CH-3010 Bern - Switzerland

^[3] Univ Sao Paulo, Ribeirao Preto Sch Med, Av Bandeirantes 3900, BR-14048900 Ribeirao Preto, SP - Brazil

^[4] Univ Sao Paulo, Ribeirao Preto Sch Dent, Av Bandeirantes 3900, BR-14048900 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Journal of Dentistry; v. 81, p. 64-69, FEB 2019.
• Web of Science Citations: 0

Abstract

Objectives: This in vivo study compared the protein profile of the acquired enamel pellicle (AEP) in volunteers 1) with gastroesophageal reflux disease (GERD) and erosive tooth wear (ETW) (BEWE >= 9; GE group); 2) with GERD without ETW (BEWE = 0; GNE group) and 3) control (without GERD and BEWE = 0; C group). Materials and methods: Twenty-four subjects (8/group) participated. AEP was formed during 120 min and collected. After protein extraction, the samples were submitted to reverse phase liquid chromatography coupled to mass spectrometry. Label-free proteomic quantification was performed using Protein Lynx Global Service software. Results: In total, 458 proteins were identified. Seventy-six proteins were common to all the groups. The proteomic profile of the AEP was quite different among the distinct groups. The numbers of proteins exclusively found in the C, GE and GNE groups were 113, 110 and 81, respectively. Most of the proteins exclusively identified in the C and GNE groups bind metals, while those in the GE group are mainly membrane proteins. Many proteins were found exclusively in the reflux groups. In the quantitative analyses, when the GNE group was compared with the GE group, the proteins with the highest decreases were Lysozyme C, Antileukoproteinase, Cathepsin G, Neutrophil defensins and Basic salivary proline-rich proteins, while those with the highest increases were subunits of Hemoglobin, Albumin and isoforms of Cystatin. Conclusion: Profound alterations in the proteomic profile of the AEP were seen in GNE compared with GE volunteers, which might play a role in the resistance to ETW seen in the first. (AU)

• FAPESP's process: 14/26606-5 - Differences in the composition of the acquired pellicle in pacients with gastroesophageal reflux disease with and without dental erosion: proteomic study
• Grantee: Tatiana Martini
• Support Opportunities: Scholarships in Brazil - Master