Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Analysis of heparanase isoforms and cathepsin B in the plasma of patients with gastrointestinal carcinomas: analytical cross-sectional study

Full text
Author(s):
Show less -
Carina Mucciolo Melo [1] ; Clarice Silvia Taemi Origassa [2] ; Thérèse Rachell Theodoro [3] ; Leandro Luongo Matos [4] ; Thaís Aguilar Miranda [5] ; Camila Melo Accardo [6] ; Rodrigo Ippolito Bouças [7] ; Eloah Rabello Suarez [8] ; Madalena Maria Nunes Silva Pares [9] ; Daniel Reis Waisberg [10] ; Giovanna Canato Toloi [11] ; Helena Bonciani Nader [12] ; Jaques Waisberg [13] ; Maria Aparecida Silva Pinhal [14]
Total Authors: 14
Affiliation:
Show less -
[1] Universidade Federal de São Paulo (Unifesp). Department of Biochemistry - Brasil
[2] Universidade Federal de São Paulo (Unifesp). Department of Biochemistry - Brasil
[3] Faculdade de Medicina do ABC (FMABC) - Brasil
[4] Faculdade de Medicina do ABC (FMABC). Department of Biochemistry - Brasil
[5] Universidade Federal de São Paulo (Unifesp). Department of Biochemistry - Brasil
[6] Universidade Federal de São Paulo (Unifesp). Department of Biochemistry - Brasil
[7] Universidade Federal de São Paulo (Unifesp). Department of Biochemistry - Brasil
[8] Universidade Federal de São Paulo (Unifesp). Department of Biochemistry - Brasil
[9] Associação Beneficente de Coleta de Sangue (Colsan) - Brasil
[10] Universidade de São Paulo (USP). Surgery Department - Brasil
[11] Faculdade de Medicina do ABC (FMABC) - Brasil
[12] Universidade Federal de São Paulo (Unifesp). Department of Biochemistry - Brasil
[13] Faculdade de Medicina do ABC (FMABC). Department of Surgery - Brasil
[14] Faculdade de Medicina do ABC (FMABC). Department of Biochemistry - Brasil
Total Affiliations: 14
Document type: Journal article
Source: São Paulo Medical Journal; v. 133, n. 1, p. 28-35, 2014-10-28.
Abstract

CONTEXT AND OBJECTIVE: Heparanase-1 degrades heparan sulfate and has been correlated with tumor progression. Although the isoform heparanase-2 has no catalytic activity, it seems to be important for modulating heparanase-1 activity. Cathepsin B is a proteinase involved in tumor metastasis. The aim of this study was to analyze heparanase isoform expression and cathepsin B activity in plasma samples from patients with gastrointestinal carcinomas, compared with healthy individuals (control group). DESIGN AND SETTING: This was an analytical cross-sectional study. Peripheral blood samples were collected at a Brazilian public hospital, from 21 patients with histopathological diagnoses of gastrointestinal carcinomas and from 43 healthy individuals. The analyses were performed in two Brazilian medical schools. METHODS: Heparanase isoforms were identified and quantified in plasma samples by means of Western blot. The enzymatic activities of heparanase-1 and cathepsin B were also measured. RESULTS: The results demonstrated that the expression of both heparanase isoforms was significantly greater in plasma samples from gastrointestinal carcinoma patients, compared with the control group. Logistic regression analysis showed that increased heparanase-1 and heparanase-2 expression was exclusively dependent on the tumor. There was a significant increase in heparanase-1 and cathepsin B activity in the patients' plasma. CONCLUSION: Overexpression of heparanase-1 and heparanase-2, along with increased heparanase-1 and cathepsin B activity in plasma, is associated with the diagnosis of gastrointestinal carcinoma. These findings provide support for using non-invasive assays (plasma samples) as an auxiliary method for diagnosing gastrointestinal tumors. (AU)

FAPESP's process: 11/18688-3 - Molecular studies of the intervertebral disc degeneration
Grantee:Maria Aparecida da Silva Pinhal
Support Opportunities: Regular Research Grants