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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

PHEMA Hydrogels Obtained by Infrared Radiation for Cartilage Tissue Engineering

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Passos, Marcele F. [1, 2] ; Carvalho, Nayara M. S. [1, 3] ; Rodrigues, Ana Amelia [1] ; Bavaresco, Vanessa P. [1, 4] ; Jardini, Andre L. [1, 3] ; Maciel, Maria Regina W. [1, 3] ; Maciel Filho, Rubens [1, 3]
Total Authors: 7
[1] Natl Inst Biofabriat INCT BIOFABRIS, Sch Chem Engn, BR-13083852 Campinas, SP - Brazil
[2] Fed Univ Para UFPA, Inst Biol Sci, Sch Biotechnol, BR-66075110 Belem, Para - Brazil
[3] State Univ Campinas UNICAMP, Lab Optimizat Design & Adv Proc Control LOPCA, Sch Chem Engn, BR-13083852 Campinas, SP - Brazil
[4] State Univ Campinas UNICAMP, CTC Plast Dept, BR-13087261 Campinas, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Web of Science Citations: 0

Although the exposure of polymeric materials to radiation is a well-established process, little is known about the relationship between structure and property and the biological behavior of biomaterials obtained by thermal phenomena at 1070nm wavelength. This study includes results concerning the use of a novel infrared radiation source (ytterbium laser fiber) for the synthesis of poly(2-hydroxyethyl methacrylate) (PHEMA) hydrogel in order to produce medical devices. The materials were obtained by means of free radical polymerization mechanism and evaluated regarding its cross-linking degree, polymer chain mobility, thermal, and mechanical properties. Their potential use as a biomaterial toward cartilage tissue was investigated through incubation with chondrocytes cells culture by dimethylmethylene blue (DMMB) dye and DNA quantification. Differential scanning calorimetry (DSC) results showed that glass transition temperature (T-g) was in the range 103 degrees C-119 degrees C, the maximum degree of swelling was 70.8%, and indentation fluency test presented a strain of 56%-85%. A significant increase of glycosaminoglycans (GAGs) concentration and DNA content in cells cultured with 40wt% 2-hydroxyethyl methacrylate was observed. Our results showed the suitability of infrared laser fiber in the free radicals formation and in the rapid polymer chain growth, and further cross-linking. The porous material obtained showed improvements concerning cartilage tissue regeneration. (AU)