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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Determination of 17 potential endocrine-disrupting chemicals in human saliva by dispersive liquid-liquid microextraction and liquid chromatography-tandem mass spectrometry

Full text
Author(s):
de Oliveira, Mariana Lepri [1] ; Rocha, Bruno Alves [1] ; de Oliveira Souza, Vanessa Cristina [1] ; Barbosa, Jr., Fernando [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Lab Toxicol & Essencialidade Met, Ave Cafe S-N, BR-14049903 Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Talanta; v. 196, p. 271-276, MAY 1 2019.
Web of Science Citations: 4
Abstract

Endocrine-disrupting chemicals are a group of emerging contaminants that alters the function of the endocrine system, causing possible adverse health effects. In this study, a dispersive liquid-liquid microextraction method coupled with liquid chromatography-mass spectrometry for the simultaneous determination of 17 potential EDCs, bisphenols (A, S, P, AP, AF, Z), parabens (methyl-, ethyl-, propyl-, butyl-, benzyl-paraben), benzophenones (3, 1, 2, 8, 4-OH BP) and triclocarban, in human saliva samples was developed. Several parameters such as, type and volume of extraction and dispersive solvents, pH sample, ionic strength and, agitation, that affect extraction efficiency were investigated. Under the optimized conditions, the matrix-matched calibration curves of all analytes presented correlation coefficients higher than 0.99 (range level of 1-20 ng mL(-1)). The intra and inter day coefficient of variation and relative standard deviation were lower than 20%, at 1 ng mL(-1). The limits of detection and quantification ranged from 0.01 to 0.15 ng mL(-1) and 0.05-0.40 ng mL(-1), respectively. Finally, the proposed method was applied in the simultaneous determination of several endocrine-disrupting chemicals classes in 10 human saliva samples In conclusion, the proposed method is an attractive alternative for application in large-scale human biomonitoring studies. (AU)

FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
Grantee:Lauro Tatsuo Kubota
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 13/23710-3 - The use of Shpol'skii spectroscopy and LC-MS/MS for determination of bisphenol A and analogues in urine samples and exposure assessment in children living in the São Paulo State, Brazil
Grantee:Bruno Alves Rocha
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 14/19914-5 - Impact of transplacental exposure to tobacco smoke in the DNA of newborn: genetic and epigenetic effects
Grantee:Fernando Barbosa Júnior
Support Opportunities: Regular Research Grants
FAPESP's process: 16/02949-6 - The development of analytical procedures for Hg and as speciation in environmental and biological samples by HPLC-ICP-MS and sample preparation with microextraction by packed sorbent (MEPS)
Grantee:Fernando Barbosa Júnior
Support Opportunities: Regular Research Grants