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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Oropouche orthobunyavirus: Genetic characterization of full-length genomes and development of molecular methods to discriminate natural reassortments

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Teixeira Nunes, Marcio Roberto [1, 2] ; de Souza, William Marciel [3, 4] ; Savji, Nazir [5] ; Figueiredo, Mario Luis [6] ; Cardoso, Jedson Ferreira [1, 7] ; da Silva, Sandro Patroca [8] ; da Silva de Lima, Clayton Pereira [1] ; Vasconcelos, Helena Baldez [8] ; Rodrigues, Sueli Guerreiro [8] ; Lipkin, W. Ian [9] ; Costa Vasconcelos, Pedro Fernando [8] ; Palacios, Gustavo [10]
Total Authors: 12
[1] Minist Hlth, Evandro Chagas Inst, Ctr Technol Innovat, Ananindeua, Para - Brazil
[2] Univ Texas Med Branch, Dept Pathol, Ctr Biodef & Emerging Infect Dis, Galveston, TX 77555 - USA
[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Virol Res Ctr, Ribeirao Preto, SP - Brazil
[4] Univ Glasgow, Ctr Virus Res, MRC, Glasgow, Lanark - Scotland
[5] Massachusetts Gen Hosp, Dept Med, Boston, MA 02114 - USA
[6] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Sao Paulo - Brazil
[7] Minist Hlth, Evandro Chagas Inst, Posgrad Program Virol, Ananindeua - Brazil
[8] Minist Hlth, Evandro Chagas Inst, Dept Arbovirol & Hemorrhag Fevers, Ananindeua - Brazil
[9] Columbia Univ, Mailman Sch Publ Hlth, Ctr Infect & Immun, New York, NY - USA
[10] US Army, Med Res Inst Infect Dis, Ctr Genome Sci, Ft Detrick, MD 21702 - USA
Total Affiliations: 10
Document type: Journal article
Source: INFECTION GENETICS AND EVOLUTION; v. 68, p. 16-22, MAR 2019.
Web of Science Citations: 0

Oropouche orthobunyavirus (OROV) has significant impact in public health in Amazon region. This arbovirus is one of the most common causes of febrile illness in Brazil, and is responsible for several epidemics since 1960's. In this study, we sequenced and characterized the complete coding sequences (S-, M- and L-RNA) of 35 OROV isolates from Brazil. Here, we classified 20 strains in genotype I from Para and Maranhao states, nine as genotype II from Para and Rondonia states confirmed, four classified into genotype III from Acre, Maranhao, Minas Gerais and Rondonia states and two genotype IV from Amazonas State. Also, we did not observe reassortment events involving the OROV isolates. In addition, we developed novel RT-PCR tools to identify reassortment events among OROV strains. These data will be useful to better understand the molecular epidemiology and diagnostic of OROV infections. (AU)

FAPESP's process: 12/24150-9 - Research of virus in wild rodents, mosquitoes and ticks
Grantee:William Marciel de Souza
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 17/13981-0 - Characterization, genomics and diagnostic of viruses with importance for public health in Brazil by high throughput sequencing
Grantee:William Marciel de Souza
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 15/05778-5 - Bioinformatics analysis of data of viral metagenomic of animals from Brazil and Antarctica
Grantee:William Marciel de Souza
Support type: Scholarships abroad - Research Internship - Doctorate