Antimicrobial photodynamic therapy improves the alveolar repair process and prevents the occurrence of osteonecrosis of the jaws after tooth extraction in senile rats treated with zoledronate

Author(s):Show less -

Ervolino, Edilson ^{[1, 2, 3]} ; Statkievicz, Cristian ^[4] ; Toro, Luan Felipe ^{[1, 3]} ; de Mello-Neto, Joao Martins ^{[4, 3]} ; Cavazana, Thamires Priscila ^[5] ; Mardegan Issa, Joao Paulo ^[6] ; Menegati Dornelles, Rita Cassia ^[2] ; de Almeida, Juliano Milanezi ^[4] ; Hitomi Nagata, Maria Jose ^[4] ; Okamoto, Roberta ^{[2, 4]} ; Casatti, Claudio Aparecido ^[2] ; Garcia, Valdir Gouveia ^{[4, 3]} ; Theodoro, Leticia Helena ^{[4, 3]}

Affiliation

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Rua Prof Dr Antonio Celso Wagner Zanin 250, BR-18618689 Botucatu, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Dent, Grp Res & Study Laser Dent, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Dept Surg & Integrated Clin, Sch Dent, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[5] Sao Paulo State Univ Unesp, Sch Dent, Dept Pediat Dent & Publ Hlth, Rua Jose Bonifacio 1193, BR-16015050 Aracatuba, SP - Brazil

^[6] Sao Paulo Univ USP, Sch Dent, Dept Morphol Physiol & Basic Pathol, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: BONE; v. 120, p. 101-113, MAR 2019.
• Web of Science Citations: 3

Abstract

This study evaluated the effects of antimicrobial photodynamic therapy (aPDT) in the alveolar repair of rats with major risk factors for bisphosphonate-related osteonecrosis of the jaws (BRONJ). Senile rats received 0.45 ml of vehicle (VEH and VEH/aPDT) or 0.45 ml of zoledronate (ZOL and ZOL/aPDT) every three days for seven weeks. After three weeks of treatment, the first lower left molar was extracted. VEH/aPDT and ZOL/aPDT were submitted to aPDT on the extraction site at 0, 2 and 4 days postoperatively. Euthanasia was performed 28 days postoperatively and the extraction site was evaluated by clinical, histological, histometric, histochemical and immunohistochemical analysis. ZOL showed tissue repair impairment; lower percentage of newly formed bone tissue (NFBT); higher percentage of non-vital bone tissue (NVBT); fewer mature collagen fibers and increased immunolabeling for tumor necrosis factor (TNF alpha), interleukin (IL)-1 beta and IL-6. ZOL/aPDT showed clinical and histological characteristics of the extraction site, percentage of NFBT and percentage of mature collagen fiber similar to VEH. Percentage of NVBT and immunolabeling for inflammatory cytokines in ZOL/aPDT was lower than in ZOL. Immunolabeling for tartarato-resistant acid phosphatase (TRAP) was lower in ZOL and ZOL/aPDT. aPDT in the dental extraction site improves tissue repair process and prevents the occurrence of BRONJ-like lesions after tooth extraction. (AU)

• FAPESP's process: 13/25367-4 - Effect of zoledronic acid during healing process of soft tissue overlying the site of tooth extraction: a study involving the main risk factors for developing osteonecrosis of the jaws
• Grantee: Thamires Priscila Cavazana
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/03067-6 - Expression of teneurins (TEN) and teneurins C-terminal associated peptides (TCAP) in the central nervous system of non-human primates (Cebus apella)
• Grantee: Cláudio Aparecido Casatti
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/26779-4 - Effect of zoledronate during the alveolar healing process in rats: study involving the major risk factors for developing osteonecrosis of the jaws and assessment of antimicrobial photodynamic therapy (aPDT) as preventive therapy
• Grantee: Edilson Ervolino
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/02199-1 - Evaluation of the effect of Antimicrobial Photodynamic Therapy (aPDT) during the alveolar healing process in rats that presents the major risk factors for developing osteonecrosis of the jaws associated with the use of bisphosphonates
• Grantee: Cristian Statkievicz
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 15/22395-2 - Evaluation of the effectiveness and safety of scaling and root planing for the treatment of experimental periodontitis in rats under therapy with oncologic dose of zoledronate
• Grantee: Luan Felipe Toro
• Support Opportunities: Scholarships in Brazil - Scientific Initiation