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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Exploring oyster mushroom (Pleurotus ostreatus) substrate preparation by varying phase I composting time: changes in bacterial communities and physicochemical composition of biomass impacting mushroom yields

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Author(s):
Vieira, F. R. [1, 2] ; Pecchia, J. A. [1] ; Segato, F. [3] ; Polikarpov, I. [4]
Total Authors: 4
Affiliation:
[1] Penn State Univ, Dept Plant Pathol & Environm Microbiol, University Pk, PA 16802 - USA
[2] Univ Estadual Paulista, Dept Engn Rural, Botucatu, SP - Brazil
[3] Univ Sao Paulo, Dept Biotecnol, Escola Engn Lorena, Lorena, SP - Brazil
[4] Univ Sao Paulo, Dept Fis & Ciencias Interdiciplinares, Inst Fis Sao Carlos, Sao Carlos, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Applied Microbiology; v. 126, n. 3, p. 931-944, MAR 2019.
Web of Science Citations: 0
Abstract

Aims To investigate characterization of the bacterial community composition and functionality and their impact on substrate biodegradation as well as mushroom yield. Methods and Results Bacterial diversity, composition and functionality were accessed by DNA-derived analysis for a sugarcane straw-based substrate composted for either 5, 10 or 15 days. In addition, carbon and nitrogen losses, carbohydrate conversion and mushroom yields were measured for the different treatments. Changes were observed in the bacterial community diversity and composition after the process started, but not during the composting process itself. Following phase I, Acinetobacter sequences were recovered in high numbers, and selected genes associated with nitrogen metabolism and lignocellulose deconstruction were mapped. Substrate physicochemical composition showed elevated carbon and nitrogen losses after 10 and 15 days of phase I with reductions in mushroom yield. Conclusions Acinetobacter species appear to play an important role in substrate degradation processes, and a 5-day phase I period showed a significant higher mushroom yield compared to composting for either 10 or 15 days. Significance and Impact of the Study: This study confers a better understanding of the bacterial community manipulation during the substrate preparation and their influence in substrate selectivity for the Pleurotus ostreatus cultivation. (AU)

FAPESP's process: 13/21545-5 - Identification and molecular studies of enzymes from thermophilic fungi Aspergillus niveus and Myceliophtora thermophila
Grantee:Fernando Segato
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/18714-2 - Enzymatic oxidation of sugarcane bagasse: discovery, characterization and new application of oxidative enzymes active in carbohydrates, applied to the enhancement of a fungal cell factory
Grantee:Fernando Segato
Support type: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
FAPESP's process: 15/13684-0 - Structural and functional studies of enzymes that participate in complex carbohydrates synthesis and degradation
Grantee:Igor Polikarpov
Support type: Research Projects - Thematic Grants
FAPESP's process: 09/52840-7 - Center of Biological and Industrial Processes for Biofuels - CeProBIO
Grantee:Igor Polikarpov
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants